599 Results for: "pcr enzymes and reagents"

Supplier: New England Biolabs (NEB)

Bst 2.0 DNA Polymerase is an in silico designed homologue of Bacillus stearo thermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment)

Supplier: New England Biolabs (NEB)

New England Biolabs supplies a unique 10X reaction buffer for use with Taq DNA Polymerase in reaction systems which require a detergent free, low ionic strength buffer.

Supplier: New England Biolabs (NEB)

VentR (exo-) DNA Polymerase has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity associated with VentR DNA Polymerase

Supplier: New England Biolabs (NEB)

Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity.

Supplier: MERIDIAN LIFE SCICNE, INC BE

MangoTaq™ DNA Polymerase is a formulation of Taq DNA Polymerase that offers high-yield across a wide range of DNA concentrations.

Supplier: New England Biolabs (NEB)

OneTaq® Hot Start 2X Master Mix with GC Buffer is an optimized blend of Taq and Deep VentR ™ DNA Polymerases combined with an aptamer-based inhibitor.

Supplier: New England Biolabs (NEB)

LongAmp Hot Start Taq DNA Polymerase is a unique blend of aptamer-based Hot Start Taq and Deep VentR™ DNA Polymerases.

Supplier: G-Biosciences

High-fidelity DNA Polymerase possesses 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This Polymerase has the fidelity, sensitivity and processivity with an error rate ~2.8×10² fold lower than Taq DNA polymerase, and significantly lower than other proofreading DNA polymerases.

Supplier: New England Biolabs (NEB)

Phusion Hot Start Flex 2X Master Mix offers robust high fidelity performance and room temperature reaction setup in a convenient master mix format

Supplier: Zymo Research

PureRec Duplex-Specific Nuclease (DSN) is an endonuclease that selectively digests double-stranded DNA (dsDNA) and DNA in DNA-RNA hybrids, exhibiting virtually no activity towards single-stranded DNA (ssDNA) or RNA.

Supplier: New England Biolabs (NEB)

The Phusion High-Fidelity PCR Kit (large) contains a sufficient supply of Phusion High-Fidelity DNA Polymerase, Phusion HF and GC Buffers, deoxynucleotides, magnesium chloride, DMSO, and DNA size standard to perform 200 reactions

Supplier: Promega Corporation

GoTaq G2 Hot Start Master Mixes are ready-to-use mixes containing GoTaq G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium

Supplier: New England Biolabs (NEB)

Q5 Hot Start High-Fidelity DNA Polymerase is a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.

Supplier: G-Biosciences

Hot start high-fidelity DNA Polymerase 2X master mix is ready to use premix which contains high fidelity DNA Polymerase, dNTPs and optimized buffer with stabilizers. The high-fidelity DNA Polymerase possesses 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities which has the fidelity, sensitivity and processivity with an error rate ~2.8×10² fold lower than Taq DNA polymerase, and significantly lower than other proofreading DNA polymerases.

Supplier: Quantabio

Superior sensitivity and multiplexing for DNA amplification of long targets.

Supplier: EMPIRICAL BIOSCIENCE

Chemically modified Taq DNA polymerase designed for reducing non-specific DNA amplification due to primer-dimer formation. FlashTaq™ is completely suppressed prior to activation and regains full enzymatic activity in only 2 min at 95 °C. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. FlashTaq™ HotStart DNA Polymerase is supplied in a storage buffer containing: HEPES, KCl, EDTA, DTT, Glycerol, and other stabilizers.

Supplier: New England Biolabs (NEB)

Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets.

Supplier: EMPIRICAL BIOSCIENCE

Phu-50 High Fidelity Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Phu DNA polymerase but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5 - 3' direction but does not possess a 5 - 3' exonuclease replacement activity. Its inherent 3 - 5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Phu-50 Polymerase-generated PCR fragments are blunt.

Supplier: Intact Genomics

Pfu 2X master mix is ready to use premix which contains Pfu DNA polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer.

Supplier: EMPIRICAL BIOSCIENCE

Chemically modified Taq DNA polymerase designed for reducing non-specific DNA amplification due to primer-dimer formation. FASTaq™ is completely suppressed prior to activation, and regains full enzymatic activity after 10 min at 95 °C. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli.

Supplier: New England Biolabs (NEB)

The Q5 Hot start high-fidelity 2X master mix features a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.

Supplier: PROMEGA SUNNYVALE INC SE

This Promega offers enhanced amplification over conventional Taq DNA polymerases. Supplied with 5X colorless reaction buffer and a 5X green buffer, which both allow for direct loading into agarose gels with no loading dye required. The included 5X green buffer allows you to visualize migration during electrophoresis. Each tube contains 100 U at 5 U/μl. Note: Requires storage at − 20°C.

Supplier: New England Biolabs (NEB)

The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets.

Supplier: Promega Corporation

GoTaq Hot Start Master Mixes are all-in-one master mixes (just add template and primers) that contain the high-performance GoTaq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 94-95°C for two

Supplier: New England Biolabs (NEB)

Q5 Blood direct 2X master mix can amplify a wide variety of targets direct from dried blood spots to up to 30% whole human blood, skipping DNA purification. The master mix includes polymerase and dNTPs in an optimized buffer with increased resistance to inhibitors in blood, anti-coagulants, and chemicals on filter papers. It is capable of amplifying products up to 7.5 kb from human whole blood cells preserved with Na EDTA, K2 EDTA, Na citrate and Na heparin as well samples stored on common preservative filter papers.

Supplier: Lucigen

Purchase individual FailSafe™ DNA polymerase and/or FailSafe™ 2X PreMix Buffers here

Supplier: Thermo Scientific Chemicals

Mycoplasma PCR Detection Kit has been used for screening of mycoplasma infection.

Supplier: New England Biolabs (NEB)

New England Biolabs supplies a 5X reaction buffer and supplemental reagent for use with Q5® High-Fidelity DNA Polymerase and Q5 Hot Start High-Fidelity DNA Polymerase.

Supplier: EMPIRICAL BIOSCIENCE

Thermo stable enzyme gene isolated from Thermus aquaticus YT1 and expressed in E. coli.

Supplier: Alkali Scientific

Achieve accurate genotyping results with Radiant™ qPCR Genotyping Mix, designed for high-performance PCR.