599 Results for: "pcr enzymes and reagents"

Supplier: New England Biolabs (NEB)

Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer.

Supplier: New England Biolabs (NEB)

ElectroLigase™ combines T4 DNA ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG.

Supplier: MP Biomedicals

Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.

Supplier: Enzo Life Sciences

Terminal labeling is an ideal procedure for the labeling of oligonucleotides with haptens such as biotin or fluorescein.

Supplier: New England Biolabs (NEB)

The ultra II DNA library prep kit for Illumina meets the challenge of constructing high quality libraries from ever-decreasing input quantities.

Supplier: Cytiva

illustra™ TempliPhi large construct DNA amplification kit (TempliPhi LC kit) is used to prepare templates for BAC or fosmid DNA sequencing.

Supplier: New England Biolabs (NEB)

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer

Supplier: Stemcell Technologies

Polymerase, buffers, and dNTPs for high-fidelity PCR amplification.

Supplier: New England Biolabs (NEB)

The Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours

Supplier: New England Biolabs (NEB)

NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly.

Supplier: New England Biolabs (NEB)

The NEBNext Ultra II RNA First Strand Synthesis Module has been optimized to convert a broad range of input amounts of RNA into cDNA using random priming.

Supplier: Corning

Sealing films are ideal for 96-well, 384-well, deep well, and other assay and PCR plates.

Supplier: New England Biolabs (NEB)

T7 DNA Polymerase catalyzes the replication of T7 phage DNA during infection

Supplier: Cytiva

The illustra™ GenomiPhi™ HY DNA Amplification Kit is part of the Phi29 DNA polymerase family of products. It contains all of the components necessary for genomic DNA preparation by isothermal strand displacement amplification. The product protocol is very simple, and provides typical yields of 40 to 50 μg DNA and average product lengths 10 kb. The starting material for GenomiPhi reactions can be purified DNA or non purified cell lysates.

Supplier: MilliporeSigma

Genomic DNAs are highly purified preparations of high molecular weight genomic DNA from a variety of organisms.

Supplier: IBI Scientific

Kit is designed to recover or concentrate DNA fragments (50bp–10kb) from agarose gel, PCR, or other enzymatic reactions

Supplier: TELESIS BIO

Generate seamless plasmid DNA or BAC clones without restriction digestion.

Supplier: New England Biolabs (NEB)

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

Supplier: MilliporeSigma

The pT7Blue cloning kit features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an EcoR V site used for T-cloning flanked by an Nde I site, which allows inserts to be conveniently subcloned into the Nde I site of many pET vectors.

Supplier: Bulldog Bio

A reverse transcriptase engineered to deliver phenomenal results.

Supplier: TELESIS BIO

Generate seamless plasmid DNA or BAC clones without restriction digestion.

Supplier: MP Biomedicals

Betaine is a quaternary amine found in bacteria and higher plants which acts as an osmoprotectant (able to restore and maintain osmotic balance in living cells) and enhances the freezing tolerance of of living organisms. This is probably due to its involvement in the protection of macrocomponents of plant cells, such as protein complexes and membranes, under stress conditions. Betaine is biosynthesized in living cells by a two-step oxidation method from choline. The primary enzyme involved in this biosynthesis is choline oxidase.

Supplier: MP Biomedicals

Glycerol is a simple polyol compound which is a viscous liquid that is widely used in pharmaceutical formulations. It has three hydroxyl groups that are responsible for its solubility in water and its hygroscopic nature.It is a trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, sweetening agent and is of low toxicity.

Supplier: IBI Scientific

The IBI high throughput 96-Well PCR Clean-Up Kit is designed to recover or concentrate DNA fragments from PCR or other enzymatic reactions using an efficient 96-well binding plate system.

Supplier: Cytiva

For the amplification of difficult templates for successful DNA sequencing in 18 hours.

Supplier: New England Biolabs (NEB)

The Exo-CIP Rapid PCR cleanup kit contains optimized formulations of thermolabile Exonuclease I and thermolabile Calf Intestinal Phosphatase (CIP) and is used to rapidly degrade residual PCR primers and dephosphorylate excess dNTPs after amplification.

Supplier: Promega Corporation

The Wizard SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100 bp to 10 kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests.

Supplier: MP Biomedicals

Ammonium sulfate is an inorganic salt prepared by treating ammonia with sulfuric acid. Ammonium sulfate decomposes upon heating above 250 °C, forming ammonium bisulfate.

Supplier: Enzo Life Sciences

The procedure for labeling of DNA probes with a polynucleotide "tail" containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions.

Supplier: Promega Corporation

TNT SP6 High-Yield Wheat Germ Protein Expression System, based on an optimized wheat germ extract, is a single-tube, coupled transcription/translation system designed to express proteins in only two hours.