242 Results for: "Tissue Culture Plastics"

Supplier: VWR International

These serological plastic pipettes are designed to use with tissue culture work.

Supplier: Thermo Fisher Scientific

These 25x75mm slides are Nunclon™Δ treated for tissue culture growth and attachment

Supplier: DWK Life Sciences (KIMBLE)

These borosilicate culture tubes are suitable for tissue culture work and general bacteriological use.

Supplier: Labconco

Formulated for use in automatic laboratory glassware washers

Supplier: MP Biomedicals

The PlantCon™ container consists of a cover and a bottom and features a vent for gas exchange and a drip control lip to recycle condensation. The cover can be snapped on tight for long-term culture or loosely fitted during acclimation to external conditions prior to transplantation. Provided presterilized, made of clear unbreakable plastic. These disposable space saving containers are specifically designed for optimal plant cell culture applications.

Supplier: Drummond

This lightweight, battery-powered pipette controller accepts plastic or glass pipettes from 1 to 100 ml.

Supplier: REVVITY HEALTH SCIENCES, INC.

Ready-to-use nucleic acid purification kit to be used with the chemagic Prepito instrument.  Based on proprietary M-PVA Magnetic Bead technology. Suitable for diverse sample material, e.g., blood, buffy coat, saliva, plasma, serum, lavage, tissue, cells, DBS, bacterial culture, and amniotic fluid. 180 preps, all plastics included.

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: MP Biomedicals

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature

Supplier: Ace Glass

This Fernbach culture flask has a long-style 38mm neck for plastic or stainless steel closures

Supplier: Ace Glass

This shaker flask has a long-style neck for use with plastic closures

Supplier: Ace Glass

This Nephelo culture flask has a long-style neck that accommodates a 25mm stainless steel or plastic closure

Supplier: Ace Glass

This Nephelo culture flask has a long-style neck that accommodates a 25mm stainless steel or plastic closure

Supplier: Foxx Life Sciences

The sterile EZlabpure™ Media Bottles, Polystyrene (PS) and PETG, have been designed specifically for Life Science researchers to store common solutions such as tissue culture media, serum, and buffers.

Supplier: Chemglass

A 114 µm thick hydrophobic porous sealing film with medical-grade adhesive for tissue culture plates, bio-blocks, and 96-well plates where gas exchange is necessary for cell or bacterial growth

Supplier: DWK Life Sciences (KIMBLE)

Standard-Tip Serological Pipettes are irradiation sterilized and suitable for tissue culture work.

Supplier: Thermo Fisher Scientific

Perform a variety of applications across a broad range of sizes and styles to suit a variety of experimental needs with Thermo Scientific™ Nunc™ Cell Culture/Petri Dishes

Supplier: NEST SCIENTIFIC

NEST cell culture plates have round edges for better handling. Low profile, low-evaporation lid design assures uniform growth.

Supplier: PROTIDE PHARMACEUTICALS

A unique cryopreservation solution that does not contain DMSO, fetal bovine serum, or animal protein. pZerve™ is ready to use and does not require any dilution or further processing.

Supplier: DWK Life Sciences (KIMBLE)

Tubes are suitable for tissue culture work and general bacteriological use.

Supplier: DWK Life Sciences (KIMBLE)

Flasks have a thick-walled, long tubing neck with a plain fire-polished finish sealed to an Erlenmeyer-shaped body

Supplier: PROTIDE PHARMACEUTICALS

Ready to use, balanced, cytoprotective, protein free solution with bio-stabilizers for short-term storage, regenerative medicine, stem cell processing and transportation of biological material. Short term storage can be achieved at 2-8°C or at ambient temperature for 24-48 hours. Does not contain any growth factors, hormones, or antibiotics. 2-8CELLsius™ can also be utilized as a cell washing solution.

Supplier: Corning

Ham's F-10 is a classical media designed to support the growth of a variety of cell types, including HeLa, Chinese Hamster Ovary (CHO), primary cells from tissue explants and various types of human diploid cells.

Supplier: Corning

Iscove's Modification of DMEM, with L-Glutamine and 25mM HEPES, without Alpha Thioglycerol and Beta Mercaptoethanol, without Sodium Bicarbonate. 10L plastic bottle. Store at 2 to 8[degree]C.

Supplier: DWK Life Sciences (KIMBLE)

Tubes are designed for use in tissue culture work and general bacteriological applications.

Supplier: Thermo Fisher Scientific

Supplier: DWK Life Sciences (KIMBLE)

GL Thread; 45-4. Cap Material; Melamine Cap with GL 45 mm thread finish. The 410136-4504 cap has an internal sealing ring for a positive seal against the thread. The 953909-0000 cap has a PTFE liner.

Supplier: Saint Gobain Performance Plastics

Highly recommended for all types of tissue culture work.

Supplier: VWR International

Cell and tissue culture flasks are ideal for cell growth and cell yields.

Supplier: Corning

Animal free formulation