922 Results for: "Molecular Biology Reagents"

Supplier: Eppendorf

Eppendorf epT.I.P.S.® Singles high-precision pipette tips offer unparalleled precision in 'Biopur®' and 'Sterile' grades, featuring individually wrapped tips for maximum sterility. Ideal for sensitive and critical lab applications where contamination control is paramount.

Supplier: Invitrogen

Thermo Scientific Pierce Premium Grade EDC is our highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.

Supplier: Promega Corporation

A silica membrane-based system for simple, rapid isolation of plasmid DNA from 1-10ml E. coli cultures. The miniprep procedure can be completed in 45 minutes or less.

Supplier: MP Biomedicals

The FastDNA™ Spin Kit for Plant and Animals Tissues quickly and efficiently isolates high quality genomic DNA from plant and animal tissues.

Supplier: MP Biomedicals

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-SMCC, No-Weigh Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).

Supplier: MP Biomedicals

Isolation of genomic DNA from plants, animals, bacteria, yeast, algae, and fungi.

Supplier: Omega Bio-Tek

The E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200µg of plasmid DNA in less than 60 minutes

Supplier: MilliporeSigma

Pellet Paint® Co-Precipitant is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids.

Supplier: Promega Corporation

The Wizard MagneSil Plasmid DNA Purification System provides a simple, reliable method for rapid isolation of plasmid DNA in a 96-well, high-throughput format.

Supplier: G-Biosciences

G-Biosciences' EZ Yeast™ Plasmid Prep kit is designed for the rapid isolation of 2µ plasmids from yeast patches or yeast grown in small liquid cultures

Supplier: Promega Corporation

A fast, simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour.

Supplier: Promega Corporation

A high-throughput 96-well format for fast, easy preparation of intact total RNA from small amounts of cell culture, tissue culture cells, tissue or whole blood.

Supplier: Promega Corporation

Bundle of 750 minipreps, vacuum manifold and free vacuum pump. Wizard Plus SV Minipreps is silica membrane-based system for simple, rapid isolation of plasmid DNA from 1-10ml E. coli cultures in 45 minutes or less.

Supplier: Promega Corporation

The Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems provide a simple and reliable method for the rapid isolation of plasmid DNA using a silica-membrane, 96-well, high-throughput format.

Supplier: Omega Bio-Tek

The Mag-Bind® Ultra-Pure Plasmid DNA 96 Kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin free plasmid DNA in high throughput format.

Supplier: Promega Corporation

A silica membrane-based system for simple, rapid isolation of plasmid DNA from 1-10ml E. coli cultures. The miniprep procedure can be completed in 45 minutes or less.

Supplier: Promega Corporation

The PolyATtract System 1000 isolates messenger RNA directly from crude cell or tissue lysates, eliminating the need for total RNA isolation.

Supplier: Promega Corporation

A silica membrane-based system for simple, rapid isolation of plasmid DNA from 1-10ml E. coli cultures. The miniprep procedure can be completed in 45 minutes or less.

Supplier: CELLTREAT Scientific Products

Non-sterile pipette tips for molecular biology, cell culture, and clinical testing where contamination from microorganisms doesn't directly impact the analytical results.

Supplier: CELLTREAT Scientific Products

Non-sterile pipette tips for molecular biology, cell culture, and clinical testing where contamination from microorganisms doesn't directly impact the analytical results.