You searched for: Electrophoresis Buffers
The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
Western Blot Stripping Buffers
Supplier: Li-Cor
Stripping and reprobing a western blot is a common laboratory method.
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Pierce™ 10X Tris-Glycine Buffer, Thermo Scientific
Supplier: Invitrogen
These space-saving stock solutions are ideal for quick preparation of standard Tris-glycine (pH 8.5) transfer buffer which is used for Western blotting.
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Pierce™ Blocker™ BSA Blocking Buffer, Thermo Scientific
Supplier: Invitrogen
Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.
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ELISA Wash Buffer (30X), Thermo Scientific
Supplier: Invitrogen
ELISA Wash Buffer is a Tris-based wash buffer for use in cytokine and other sandwich ELISA procedures. The buffer is the same formulation that is supplied with most Thermo Scientific Pierce ELISA Kits for cytokines and chemokines. The 30X concentrated stock solution is easily diluted 1:30 (vol:vol) in ultrapure water to yield sufficient wash buffer for a typical 96-well microplate ELISA protocol. Supplement an existing ELISA Kit or use as a convenient general purpose wash buffer for customized ELISA methods.
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ELISA Blocker Blocking Buffer, Thermo Scientific, Thermo Scientific
Supplier: Invitrogen
ELISA Blocker Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and routinely used for sandwich ELISA procedures involving cytokines, such as many of the Thermo Scientific Pierce ELISA Kits.
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Borate Buffered Saline Milk Blocking Solution-10X, BioFX®, Surmodics IVD, Inc.
Supplier: Surmodics
BioFX® Borate Buffered Saline (BBS) Milk Blocking Solution-10X Concentrate contains Non-Fat Dry Milk (NFDM) in a BBS solution that is supplied as a solution ready for dilution to specific application requirements.
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Endo Trap® HD Buffer Kit, BioVendor
Supplier: BioVendor
EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.
The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates.
EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, the High-Definition Matrix is also available for small-scale purification as pre-filled columns.
Endotoxin removal with EndoTrap® HD works effectively in the pH range of 4-9 and NaCl concentrations of 50-600 mM. Buffers such as HEPES, TRIS, MOPS, MES, and PIPES are recommended. Citrate buffers and chelators of divalent cations (like EDTA) have to be avoided
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Pierce™ Nucleic Acid Detection Blocking Buffer, Thermo Scientific
Supplier: Invitrogen
Nucleic Acid Detection Blocking Buffer is intended for use with the Thermo Scientific Chemiluminescent Nucleic Acid Detection Module Kit, of which it is also a component
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Pierce™ LDS Sample Buffer, Non-Reducing (4X), Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce LDS Sample Loading Buffer (4X) is a nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.
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Western Blot Strip-it™ Buffer, Advansta
Supplier: ADVANSTA INC MS
Stripping buffer for stripping antibodies from chemiluminescent Western blots.
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CellPro™ Western Blot Blocking Buffer, Sterile Filtered
Supplier: Alkali Scientific
Achieve reliable protein blocking in western blotting with CellPro™ western blot blocking buffer, provided sterile filtered for consistency.
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Pierce™ SuperBlock™ Dry Blend Blocking Buffer, Thermo Scientific
Supplier: Invitrogen
SuperBlock (TBS) Blocking Buffer is an optimised TBS solution containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western Blot Analysis.
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Restore™ Western Blot Stripping Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Restore™ Western Blot Stripping Buffer safely and effectively removes primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
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FLASHBlot™ Transfer Buffer, Advansta
Supplier: ADVANSTA INC MS
Transfer buffer designed for faster, more efficient protein transfer with increased sensitivity.
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Blocking Agent, Instant Nonfat Immunoblotting Grade Dry Milk
Supplier: Alkali Scientific
Achieve optimal blocking in immunoblotting with blocking agent, instant nonfat immunoblotting grade dry milk, providing rapid and efficient blocking.
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StabilCoat® BSA ELISA Stabilizer/Blocker, Surmodics
Supplier: Surmodics
StabilCoat® Immunoassay Stabilizer, and the 5X concentrate, effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. The product can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper.
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StabilBlot™ Casein Blocker-5X, TBS, BioFX®, Surmodics IVD, Inc.
Supplier: Surmodics
The StabilBlot™ Blocker Family offers a range of options, both protein-based and synthetic-based, to block non-specific binding as well as enhance sensitivity when used as a blocker and antibody diluent.
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Pierce BupH™ Dry Blend Buffers, Washing, Thermo Scientific
Supplier: Invitrogen
BupH™ packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.
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SuperBlock™ T20 (PBS) Blocking Buffer, Thermo Scientific
Supplier: Invitrogen
SuperBlock™ Blocking Buffer is optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.