You searched for: Western Blotting Reagents
Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
FLASHBlot™ Transfer Buffers, Advansta
Supplier: Advansta
Increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
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Pierce Miser™ Western Blotting Detection Reagents, Antibody Extender Solution NC, Thermo Scientific
Supplier: Invitrogen
Pierce Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.
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Restore™ Fluorescent Western Blot Stripping Buffer, Thermo Scientific
Supplier: Invitrogen
The Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.
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SuperSignal™ West HisProbe Kit, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific SuperSignal West HisProbe Kit contains HisProbe-HRP Conjugate, as well as reagents required for detection of polyhistidine-tagged protein by Western blotting
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BioFX® Chemiluminescent Ultra Sensitive HRP Membrane Substrate, Surmodics IVD, Inc.
Supplier: Surmodics
BioFX® Chemiluminescent Ultra Sensitive HRP Membrane Substrate is a luminol based formulation for western blot applications.
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BioFX® Chemiluminescent Ultra Sensitive HRP Microwell Substrate, Surmodics IVD, Inc.
Supplier: Surmodics
Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in Western Blot assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the luminol substrate, antibody/antigen titers and blockers.
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Pierce 1-Step™ Chloronaphthol Substrate Solution, Thermo Scientific
Supplier: Invitrogen
The 1-Step™ chloronaphthol solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.
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Pierce™ Guardian™ Peroxidase Conjugate Stabilizer/Diluent, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Guardian Peroxidase Conjugate Stabilizer/Diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long-term storage.
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Pierce™ LightShift™ Chemiluminescent RNA EMSA Kit, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
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Pierce 1-Step™ NBT/BCIP plus Suppressor Substrate Solution, Thermo Scientific
Supplier: Invitrogen
The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
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BrightStar™ Duration HRP Chemiluminescent Substrates
Supplier: Alkali Scientific
Achieve sensitive detection in chemiluminescence assays with the BrightStar™ Duration HRP Chemiluminescent Substrate, designed for accurate and efficient protein detection.
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Clarity™ Western ECL Substrate, Chemiluminescent Detection, Bio-Rad
Supplier: Bio-Rad
The Bio-Rad Clarity™ Western ECL Substrate, for chemiluminescent western blot detection, is compatible with any HRP-conjugate secondary antibody and is compatible with both digital and film-based imaging.
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BioFX® Chemiluminescent Ultra Sensitive AP Microwell and/or Membrane Substrate (450 nm), Surmodics
Supplier: Surmodics
Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and membrane assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the dioxetane substrate, antibody/antigen titers and blockers. Surmodics provides multiple 1-component AP chemiluminescent substrates with various intensities and wavelength emissions to allow the assay developer to choose the appropropriate substrate for the application.
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Pierce™ CN/DAB Substrate Kit, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.
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Pierce™ 1-Step™ Ultra TMB-Blotting Solution, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.
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WesternBright ECL HRP Substrate, Advansta
Supplier: ADVANSTA INC MS
Advansta's HRP substrate is the best choice for routine, film-imaged chemiluminescent Western blots.
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BioFX® Chemiluminescent Sensitive Plus HRP Microwell and/or Membrane Substrate, Surmodics IVD
Supplier: Surmodics
Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and Western Blot assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the luminol substrate, antibody/antigen titers and blockers. Surmodics provides multiple 2-component HRP chemiluminescent substrates with various intensities to allow the assay developer to choose the appropropriate substrate for the application.
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Pierce 1-Step™ TMB-Blotting Substrate Solution, Thermo Scientific
Supplier: Invitrogen
The 1-Step™ TMB-blotting solution is a blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blot and IHC experiments.
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HisProbe™-HRP Conjugate, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific HisProbe-HRP is a nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.
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Pierce™ LightShift® Chemiluminescent EMSA Kit, Thermo Scientific
Supplier: Invitrogen
LightShift® Chemiluminescent EMSA Kit is robust and sensitive system for performing electrophoretic mobility shift assays (EMSAs) to identify and characterize protein:DNA binding interactions.
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Pierce 1-Step™ NBT/BCIP Substrate Solution, Thermo Scientific
Supplier: Invitrogen
The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
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Pierce™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) HRP substrate for picogram to high-femtogram level detection.
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Pierce QuantaBlu™ NS/K Fluorogenic Substrate Kit, Thermo Scientific
Supplier: Invitrogen
QuantaBlu™ NS/K fluorogenic substrate kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays
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BioFX® DAB Membrane Substrate, Surmodics
Supplier: Surmodics
DAB membrane substrate is supplied as a two component liquid substrate system designed for the rapid detection of peroxidase in membrane applications.
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Femto HRP Chemiluminescent Substrate Kits
Supplier: Alkali Scientific
Optimize your chemiluminescent assays with the Femto HRP Chemiluminescent Substrate Kits, providing sensitive detection for various applications.
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Alkaline Phosphatase (APP) Chromogen Substrates for IHC, Vector Laboratories
Supplier: Vector Laboratories
Vector Laboratories alkaline phosphatase (AP) substrate options include standard substrates and ImmPACT substrates with higher sensitivity. The range of vibrant colors facilitates multiplex staining and improved contrast with pigmented tissues and counterstains.
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Afyon SDS-PAGE Sample Preparation Kit, Advansta
Supplier: ADVANSTA INC MS
Kit for fast, easy concentration and purification of samples for SDS-PAGE.
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WesternBright Sirius HRP Substrate, Advansta
Supplier: ADVANSTA INC MS
Sirius is the most sensitive chemiluminescent substrate from Advansta.
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Pierce™ LightShift™ Poly (dI-dC), Thermo Scientific
Supplier: Invitrogen
LightShift poly(dI-dC) can be used as competitor for nonspecific DNA binding proteins.
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Pierce™ HRP Substrates for ELISA, OPD (o-phenylenediamine dihydrochloride), Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers.