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Electrophoresis Reagents

Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

VWR® Destaining Bags

VWR® Destaining Bags

Supplier: VWR

Bags safely remove ethidium bromide from solution during overnight treatment

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VWR® Visiglo™ & Visiglo Plus™ Chemiluminescent Substrate Kits

VWR® Visiglo™ & Visiglo Plus™ Chemiluminescent Substrate Kits

Supplier: VWR

VisiGlo HRP and VisiGlo Plus HRP are luminol-based chemiluminescent substrates designed for the rapid and sensitive detection of peroxidase-labeled conjugates.

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VWR® DNA Ladders

VWR® DNA Ladders

Supplier: VWR International

DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

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VWR® DNA Ladders

VWR® DNA Ladders

Supplier: VWR International

DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

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Protein Free Blot Blocking Buffer, Azure Biosystems

Protein Free Blot Blocking Buffer, Azure Biosystems

Supplier: Azure Biosystems

Azure Protein Free Blot Blocking Buffer is a protein free blocking solution, optimized for use with fluorescent Western blots, and also an excellent blocking reagent for chemiluminescent Western blotting.

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VWR® DNA Molecular Weight Markers, 100 bp Ladder

VWR® DNA Molecular Weight Markers, 100 bp Ladder

Supplier: VWR

13 fragments ranging from 100 to 3000 bp for easy band identification.

    
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VWR®  RiboReady™ Color RNA Ladders

VWR® RiboReady™ Color RNA Ladders

Supplier: VWR

RiboReady™ Color Micro RNA Ladder is made for precise sizing of RNA molecules in denaturing acrylamide gels

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Lambda Markers

Lambda Markers

Supplier: Avantor

These DNA markers are used to determine size of unknown DNA fragments. If using within 2–3 days, they do not need to be refrigerated and can be stored right on a shelf. If storing for longer time periods, freezing is recommended.

Ship date is required.

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Radiance Plus Chemiluminescent Substrate, Azure Biosystems

Radiance Plus Chemiluminescent Substrate, Azure Biosystems

Supplier: Azure Biosystems

Radiance Plus is the most sensitive HRP substrate for chemiluminescent western blotting available from Azure.

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Fluorescent Wash Buffer, Azure Biosystems

Fluorescent Wash Buffer, Azure Biosystems

Supplier: Azure Biosystems

Azure IR Fluorescent Blot Washing Buffer is optimized for use with visible and infrared fluorescent Western blotting.

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VWR® AMRESCO EZ-Vision® DNA Ladder 1 kb

Supplier: VWR

A pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualization dye that can be applied directly to the gel.

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VWR® PCR DNA Marker

VWR® PCR DNA Marker

Supplier: VWR

8 fragments ranging from 50 to 2000 bp for easy band identification

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VWR® DNA Molecular Weight Markers, 1 kb Ladder

VWR® DNA Molecular Weight Markers, 1 kb Ladder

Supplier: VWR

11 fragments ranging from 500 to 10,000 bp for easy band identification

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VWR® DNA Molecular Weight Marker, High Range

VWR® DNA Molecular Weight Marker, High Range

Supplier: VWR

10 fragments ranging from 1,503 to 48,502 bp for easy band identification

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Radiance Q Chemiluminescent Substrate, Azure Biosystems

Radiance Q Chemiluminescent Substrate, Azure Biosystems

Supplier: Azure Biosystems

Radiance Q chemiluminescent substrate sets the bar for sensitivity and quantitative ability

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CyDye™ Mono-Reactive Maleimide Fluorescent Dyes, Cytiva

CyDye™ Mono-Reactive Maleimide Fluorescent Dyes, Cytiva

Supplier: Cytiva

CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.

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Blot Washing Buffer, Azure Biosystems

Blot Washing Buffer, Azure Biosystems

Supplier: Azure Biosystems

Azure Blot Washing Buffer can be used with any Western blotting system.

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VWR® Ready Ladder™ 50 bp DNA Marker

VWR® Ready Ladder™ 50 bp DNA Marker

Supplier: VWR

10 fragments ranging from 50 to 500 bp for easy band identification

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Transfer Buffer, Azure Biosystems

Transfer Buffer, Azure Biosystems

Supplier: Azure Biosystems

Azure’s proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

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DeStreak Rehydration Reagent, Cytiva

DeStreak Rehydration Reagent, Cytiva

Supplier: Cytiva

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

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Ethidium Bromide Greenbag™ Disposal Kit, MP Biomedicals

Ethidium Bromide Greenbag™ Disposal Kit, MP Biomedicals

Supplier: MP Biomedicals

Each EtBr Green BagTM contains a standard amount of activated material that will safely and easily concentrate Ethidium Bromide from large liquid quantities

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HIGHDEF® DAB Chromogen/Substrate Set, Enzo Life Sciences

Supplier: Enzo Life Sciences

Used in conjunction with peroxidase-based immunostaining systems.

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Pierce™ Firefly Signal Enhancer (100X), Thermo Scientific

Pierce™ Firefly Signal Enhancer (100X), Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce Firefly Signal Enhancer is designed to complement the Pierce Firefly Luciferase Flash and Glow Assay and is recommended when maximum sensitivity is desired.

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Pierce Erase-It® Background Eliminator Kit, Thermo Scientific

Pierce Erase-It® Background Eliminator Kit, Thermo Scientific

Supplier: Invitrogen

The background eliminator reduces background signal evenly over any film within minutes

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Amersham ECL Direct Labeling System, Cytiva

Amersham ECL Direct Labeling System, Cytiva

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

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Acridinium Ester, (Trigger Solution), Enzo Life Sciences

Supplier: Enzo Life Sciences

Species Independent

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HL60 Cell Extract (Non-induced), Enzo Life Sciences

HL60 Cell Extract (Non-induced), Enzo Life Sciences

Supplier: Enzo Life Sciences

Whole-cell extract of human HL-60 leukemia cells.

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Chemiluminescent HRP Substrate (membrane), Abcam

Supplier: Abcam

A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.

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Amersham™ ECL™ Prime Blocking Reagent

Amersham™ ECL™ Prime Blocking Reagent

Supplier: Cytiva

Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

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Pierce™ Substrate for Western Blotting, ECL Plus, Thermo Scientific

Pierce™ Substrate for Western Blotting, ECL Plus, Thermo Scientific

Supplier: Invitrogen

The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

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