Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
VWR® Destaining Bags
Supplier: VWR
Bags safely remove ethidium bromide from solution during overnight treatment
Expand 1 Items
VWR® Visiglo™ & Visiglo Plus™ Chemiluminescent Substrate Kits
Supplier: VWR
VisiGlo HRP and VisiGlo Plus HRP are luminol-based chemiluminescent substrates designed for the rapid and sensitive detection of peroxidase-labeled conjugates.
Expand 1 Items
VWR® DNA Ladders
Supplier: VWR International
DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.
Expand 3 Items
VWR® DNA Ladders
Supplier: VWR International
DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.
Expand 3 Items
Protein Free Blot Blocking Buffer, Azure Biosystems
Supplier: Azure Biosystems
Azure Protein Free Blot Blocking Buffer is a protein free blocking solution, optimized for use with fluorescent Western blots, and also an excellent blocking reagent for chemiluminescent Western blotting.
Expand 1 Items
VWR® DNA Molecular Weight Markers, 100 bp Ladder
Supplier: VWR
13 fragments ranging from 100 to 3000 bp for easy band identification.
Expand 2 Items
VWR® RiboReady™ Color RNA Ladders
Supplier: VWR
RiboReady™ Color Micro RNA Ladder is made for precise sizing of RNA molecules in denaturing acrylamide gels
Expand 2 Items
Lambda Markers
Supplier: Avantor
These DNA markers are used to determine size of unknown DNA fragments. If using within 2–3 days, they do not need to be refrigerated and can be stored right on a shelf. If storing for longer time periods, freezing is recommended.
Ship date is required.
Expand 4 Items
Radiance Plus Chemiluminescent Substrate, Azure Biosystems
Supplier: Azure Biosystems
Radiance Plus is the most sensitive HRP substrate for chemiluminescent western blotting available from Azure.
Expand 1 Items
Fluorescent Wash Buffer, Azure Biosystems
Supplier: Azure Biosystems
Azure IR Fluorescent Blot Washing Buffer is optimized for use with visible and infrared fluorescent Western blotting.
Expand 1 Items
VWR® AMRESCO EZ-Vision® DNA Ladder 1 kb
Supplier: VWR
A pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualization dye that can be applied directly to the gel.
Expand 1 Items
VWR® PCR DNA Marker
Supplier: VWR
8 fragments ranging from 50 to 2000 bp for easy band identification
Expand 1 Items
VWR® DNA Molecular Weight Markers, 1 kb Ladder
Supplier: VWR
11 fragments ranging from 500 to 10,000 bp for easy band identification
Expand 1 Items
VWR® DNA Molecular Weight Marker, High Range
Supplier: VWR
10 fragments ranging from 1,503 to 48,502 bp for easy band identification
Expand 1 Items
Radiance Q Chemiluminescent Substrate, Azure Biosystems
Supplier: Azure Biosystems
Radiance Q chemiluminescent substrate sets the bar for sensitivity and quantitative ability
Expand 1 Items
CyDye™ Mono-Reactive Maleimide Fluorescent Dyes, Cytiva
Supplier: Cytiva
CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.
Expand 2 Items
Blot Washing Buffer, Azure Biosystems
Supplier: Azure Biosystems
Azure Blot Washing Buffer can be used with any Western blotting system.
Expand 1 Items
VWR® Ready Ladder™ 50 bp DNA Marker
Supplier: VWR
10 fragments ranging from 50 to 500 bp for easy band identification
Expand 1 Items
Transfer Buffer, Azure Biosystems
Supplier: Azure Biosystems
Azure’s proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.
Expand 1 Items
DeStreak Rehydration Reagent, Cytiva
Supplier: Cytiva
The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.
Expand 2 Items
Ethidium Bromide Greenbag™ Disposal Kit, MP Biomedicals
Supplier: MP Biomedicals
Each EtBr Green BagTM contains a standard amount of activated material that will safely and easily concentrate Ethidium Bromide from large liquid quantities
Expand 1 Items
HIGHDEF® DAB Chromogen/Substrate Set, Enzo Life Sciences
Supplier: Enzo Life Sciences
Used in conjunction with peroxidase-based immunostaining systems.
Expand 1 Items
Pierce™ Firefly Signal Enhancer (100X), Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Firefly Signal Enhancer is designed to complement the Pierce Firefly Luciferase Flash and Glow Assay and is recommended when maximum sensitivity is desired.
Expand 1 Items
Pierce Erase-It® Background Eliminator Kit, Thermo Scientific
Supplier: Invitrogen
The background eliminator reduces background signal evenly over any film within minutes
Expand 1 Items
Amersham ECL Direct Labeling System, Cytiva
Supplier: Cytiva
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Expand 1 Items
Acridinium Ester, (Trigger Solution), Enzo Life Sciences
Supplier: Enzo Life Sciences
Species Independent
Expand 1 Items
HL60 Cell Extract (Non-induced), Enzo Life Sciences
Supplier: Enzo Life Sciences
Whole-cell extract of human HL-60 leukemia cells.
Expand 1 Items
Chemiluminescent HRP Substrate (membrane), Abcam
Supplier: Abcam
A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.
Expand 1 Items
Amersham™ ECL™ Prime Blocking Reagent
Supplier: Cytiva
Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.
Expand 1 Items
Pierce™ Substrate for Western Blotting, ECL Plus, Thermo Scientific
Supplier: Invitrogen
The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.