You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Human c-MET/HGFR Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's c-MET/hepatocyte growth factor receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against c-MET (HGFR).
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SensoLyte® 520 Thiol Quantitation Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
Thiols, compounds that contain sulfhydryl group, are powerful reducing agents capable of acting as antioxidants, enzyme cofactors, and neuromodulators.
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SensoLyte® Total GSH Assay Kit (Colorimetric), AnaSpec
Supplier: Anaspec
Glutathione (GSH) is an important antioxidant molecule that functions as a cofactor for a variety of enzymes and is involved in various biological processes
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Human AP-1 Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's activator protein-1 (AP-1) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against AP-1.
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SensoLyte® 490 MMP-1 Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec
MMP-1 (interstitial collagenase, fibroblast collagenase) is an extracellular protease
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Cyn Monkey Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's Cyn monkey progesterone receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against PGR (NR3C3).
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Pierce™ APTS (3-aminopropyltriethoxysilane), Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce APTS (3-aminopropyltriethoxysilane) is a silylation reagent for coating glass and silica surfaces to add primary amines, which can be used to crosslink and immobilize proteins and other molecules.
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SensoLyte® 520 Cathepsin B Assay Kit Fluorimetric, AnaSpec, Inc.
Supplier: Anaspec
Cathepsin B is a member of the cathepsin family consisting of 12 cysteine proteases with broad exo- and endopeptidase activity
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SensoLyte® Anti-Rat MOG (1-125) IgG Quantitative ELISA Kit, AnaSpec
Supplier: Anaspec
This kit is optimized to detect anti-rat MOG (1-125) IgG. Wells are pre-coated with recombinant rat MOG (1-125) protein and pre-blocked with BSA. The amount of anti-rat MOG IgG in serum or cerebrospinal fluid is quantified using ELISA. Ample materials and reagents are provided to perform 96 assays.
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SensoLyte® 520 BACE2 Activity Assay (Fluorimetric), AnaSpec
Supplier: Anaspec
BACE2 (β-Secretase-2, Memapsin-1) belongs to the family of transmembrane aspartic proteases
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SensoLyte® ADHP Hydrogen Peroxide Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec
Hydrogen peroxide is involved in a number of biological events, in particular, free radical-induced biochemical reactions.
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SensoLyte® 490 HCV Protease Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec
HCV protease is identified as an important drug-screening target.
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SensoLyte® 520 Cathepsin D Assay Kit Fluorimetric, AnaSpec, Inc.
Supplier: Anaspec
Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown
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SensoLyte® 520 TEV Activity Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins
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SensoLyte® Red Protease Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
The SensoLyte® Red Protease Assay Kit is widely used for continuous detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Compared to the SensoLyte® Green Protease Assay Kit, this kit contains a red fluorescent casein derivative. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly red fluorescent dye-labeled peptides (Ex/Em=546/575 nm). The increase in fluorescence intensity is directly proportional to protease activity. The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases.
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Pierce™ Cypridina Luciferase Flash Assay Kit, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
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SensoLyte® 490 HIV Protease Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec
HIV protease (PR) is identified as an important drug-screening target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics.
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SensoLyte® Anti-PLP (139-151) IgG Quantitative ELISA Kit (Mouse) (Colorimetric), AnaSpec
Supplier: Anaspec
SensoLyte® Anti-PLP (139 - 151) IgG Quantitative ELISA Kit (Mouse) is optimized to detect mouse anti-PLP (139-151) IgG. This kit is useful to researchers who wish to determine the amount of anti-PLP (139-151) antibody present in biological samples, and can help provide information on the role it plays in the development and treatment of EAE, an animal model for MS pathogenesis. Wells are pre-coated with PLP (139-151) peptide and pre-blocked with BSA. The amount of anti-PLP (139-151) IgG in mouse serum or cerebrospinal fluid is quantified using ELISA (Abs=450 nm). Ample materials and reagents are provided to perform 96 assays.
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SensoLyte® Anti-PLP (178-191) IgG Quantitative ELISA Kit (Mouse) (Colorimetric), AnaSpec
Supplier: Anaspec
SensoLyte® Anti-PLP (178-191) IgG Quantitative ELISA Kit (Mouse) is optimized to detect mouse anti-PLP (178-191) IgG. This kit is useful to researchers who wish to determine the amount of anti-PLP (178-191) antibody present in biological samples, and can help provide information on the role it plays in the development and treatment of EAE, an animal model for MS pathogenesis. Wells are pre-coated with PLP (178-191) peptide and pre-blocked with BSA. The amount of anti-PLP (178-191) IgG in mouse serum or cerebrospinal fluid is quantified using ELISA (Abs=450 nm). Ample materials and reagents are provided to perform 96 assays.
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SensoLyte® 520 MMP Profiling Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components
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Human GIPR Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's gastric inhibitory polypeptide receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against GIPR.
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Human ADRB3 Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's adrenoreceptor beta 3 (ADRB3) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRB3.
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Human IR-B Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's insulin receptor, isoform B (IR-B) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against IR-B.
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Human AVPR1A Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's arginine vasopressin receptor 1A (AVPR1A) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against AVPR1A.
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SensoLyte® Thioflavin T β-Amyloid (1-40) Aggregation Kit, AnaSpec
Supplier: Anaspec
The SensoLyte® ThT β-Amyloid (1-40) Aggregation kit provides a convenient and standard method to measure Aβ40 aggregation using Thioflavin T dye
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Human GM-CSFR Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's granulocyte-macrophage colony-stimulating factor receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against GM-CSFR (CSF2R).
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SensoLyte® Anti-Mouse/ Rat β-Amyloid (1-40) Quantitative ELISA (Colorimetric), AnaSpec
Supplier: Anaspec
This SensoLyte® high-sensitivity (2pg/mL) beta-Amyloid (1-40) Quantitative ELISA Kit (Mouse/Rat) provides a convenient and quantitative assay for determining mouse/rat beta-Amyloid (1-40) (Aβ40) amount in cell and tissue lysate as well as in body fluids
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Rabbit PGR Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's rabbit progesterone receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against PGR (NR3C3).
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SensoLyte® Plus 520 MMP-9 Assay Kit (Fluorimetric and Enhanced Selectivity), AnaSpec Inc.
Supplier: Anaspec
The SensoLyte® Plus 520 MMP-9 Assay Kit is designed for specifically detecting MMP-9 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-9 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL®520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-9-induced cleavage of the FRET substrate. Ample materials are provided to perform 96 assays in a 96-well format.
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SensoLyte® Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit, AnaSpec
Supplier: Anaspec
This kit is optimized to detect anti-human MOG (1-125) IgG in mouse serum or cerebrospinal fluid. Wells are pre-coated with recombinant human MOG (1-125) protein and pre-blocked with BSA. The amount of anti-human MOG IgG in serum or cerebrospinal fluid is quantified using ELISA. Mouse anti-human MOG IgG standard is included. Ample materials and reagents are provided to perform 96 assays in a 96-well plate format.