You searched for: Protein Linking and Labeling Systems

Supplier: Stemcell Technologies

Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

Supplier: Invitrogen

Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (biotin-N4-CTP) onto the 3' end of DNA strands where the label will be less likely to interfere with hybridization or sequence-specific binding of proteins.

Supplier: Abnova

Fluorescence Dye 488-I conjugates are significantly brighter than fluorescein conjugates and much more photostable.

Supplier: Invitrogen

The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 - 145 nm in excitation/emission wavelengths.

Supplier: Invitrogen

Thermo Scientific Pierce Maleimide Activated Horseradish Peroxidase is for preparation of HRP conjugates with proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

Supplier: BIOPOINT USA INC MS

EasyStain™ is an immunofluorescence reagent designed for use in testing water samples for the presence of Cryptosporidium oocysts and Giardia cysts.

Supplier: Cell Biolabs

PhosphoBLOCKER™ Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio

Supplier: Anaspec

Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivity to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels.

Supplier: Invitrogen

Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatized biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4.5 - 5 and requires just minutes to complete.

Supplier: Anaspec

TAMRA is one of the most popular fluorophores used in various bioconjugations. 5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates.

Supplier: Invitrogen

The Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP provides biotinylation reagents designed specifically for the labeling of primary antibodies used in immunoprecipitation applications.Optimized for IP—reagents and protocols developed specifically to address antibody labeling for immunoprecipitation applicationsEasy to use—kit configuration specifically optimized to accommodate 50–200 µg of antibody in a 100 µL reaction; desalting columns are provided to remove excess biotinylation reagent after labelingAmine reactive—reacts with primary amines (-NH2) of antibodiesEnhanced solubility—pegylation improves water solubility of the biotinylated antibody and prevents aggregation upon storageReduced steric hindrance—longer spacer arm (29 angstroms) minimize steric hindrance when binding to avidin moleculesThe Pierce Antibody Biotinylation Kit reagents have been optimized and validated to biotin label antibodies for IP and co-IP reactions

Supplier: List Biological Laboratories, Inc.

The potent toxicity of both the botulinum neurotoxins and anthrax lethal toxin is due to a zinc-dependent proteolytic activity associated with the toxins.  Measurement of this enzymatic activity provides for both a potentially sensitive and direct means for detection of the toxin, and a method for identifying potential toxin inhibitors using high throughput screening. A highly efficient approach for monitoring enzymatic activity is based on the use of fluorescence resonance energy transfer (FRET) substrates. These fluorogenic peptides contain a donor fluorescent group at one end and a suitable chromogenic acceptor group at the other.  The fluorescence is quenched initially by intramolecular energy transfer between the donor/acceptor pair.  Cleavage of the FRET substrate by the appropriate enzyme releases the fluorophore and full fluorescence is restored.  The increase in fluorescence intensity is directly proportional to the amount of enzyme present.  Enzymatic activity can be monitored continuously by recording the increase in fluorescence intensity with time.  The change in the relative fluorescence units (RFU) as cleavage occurs can be converted to nmoles of cleaved substrate from a standard curve generated using a Calibration Peptide which is the cleaved substrate containing only the N-terminally attached fluorophore.  For Botulinum neurotoxin type A, a Control FRET peptide substrate that is not cleaved by the neurotoxin but contains all remaining non-specific sites in the sequence can be used to screen background cleavage of the substrate that can occur in complex matrices.

Supplier: Invitrogen

Label peptides, antibodies and other proteins at primary amines with the coumarin-based, 405 laser-detectable Thermo Scientific DyLight™ Blue-emitting Specialty Dye

Supplier: Anaspec

The AnaTag™ B-PE Labeling Kit is optimized for use in the conjugation of B-Phycoerythrin (B-PE) to antibodies. B-PE, a fluorescent protein from phycobiliprotein family, has a primary absorption peak at 545 nm with a secondary peak at 563 nm and maximum emission at 578 nm.

Supplier: Invitrogen

Thermo Scientific™ Pierce Fluorescence Biotin Quantitation Kit requires only ten microliters of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.

Supplier: Invitrogen

Photoactivatable Biotin, Biotin-LC-ASA, Psoralen-PEG3-Biotin and TFPA-PEG3-Biotin each contain a photoactivatable group. When exposed to UV light, they become activated and insert non specifically into nearby molecules. These reagents may be used to label proteins and peptides, but they are also useful in labeling DNA, RNA and other molecules that do not contain any readily reactive functional groups.

Supplier: Invitrogen

The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.They insert an unbreakable molecular bridge between two proteins with easy-to-control stepwise coupling.

Supplier: Vector Laboratories

Fluorescein labeled Lycopersicon Esculentum (Tomato) Lectin (LEL, TL). Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Supplier: Invitrogen

The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Streptavidin) provides mass spectrometry-friendly reagents and an optimized protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.MS-compatible—reagents directly compatible with in-solution peptide digestion, enriched samples contain minimal detergent residuals detected using LC-MSSensitive—procedure successfully enriches low abundance proteins (low ng range)Low background—binding, wash, and elution buffers optimized to minimize enrichment of background proteinsReduced antibody contamination—antibody biotinylation reduces contamination in the eluate when compared to other IP methodsRobust—procedure and reagents have been robustly tested with numerous targets to ensure consistent enrichment of low abundant proteins (ng range) with at least 2 peptides identified per proteinThe Pierce MS-Compatible Magnetic IP Kit (Streptavidin) uses high-quality Pierce Streptavidin Magnetic Beads and optimized buffers that are compatible with downstream LC-MS sample preparation and analysis