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619 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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EZ-Link™ Desthiobiotinylation and Pull-Down Kit and Reagents, Thermo Scientific

EZ-Link™ Desthiobiotinylation and Pull-Down Kit and Reagents, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific EZ-Link™ Desthiobiotinylation and Pull-down Kit is a complete set of reagents to biotin-label purified proteins and use them to purify interaction complexes using streptavidin agarose resin with mild elution conditions.

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Pierce™ NHS-Fluorescein Antibody Labeling Kit, Thermo Scientific

Pierce™ NHS-Fluorescein Antibody Labeling Kit, Thermo Scientific

Supplier: Invitrogen

Pierce™ NHS-Fluorescein Antibody Labeling Kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

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Pierce™ EZ-Link™ Azide Reactive Biotinylation Reagents, Thermo Scientific

Pierce™ EZ-Link™ Azide Reactive Biotinylation Reagents, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce EZ-Link Phosphine-PEG3-Biotin is a biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies.

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GloCell™ Fixable Viability Dye Red 710

GloCell™ Fixable Viability Dye Red 710

Supplier: Stemcell Technologies

Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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Pierce™ Biotin 3' End DNA Labeling Kit, Thermo Scientific

Pierce™ Biotin 3' End DNA Labeling Kit, Thermo Scientific

Supplier: Invitrogen

Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (biotin-N4-CTP) onto the 3' end of DNA strands where the label will be less likely to interfere with hybridization or sequence-specific binding of proteins.

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Fluorescent Dye 488-I Phalloidin, Abnova

Supplier: Abnova

Fluorescence Dye 488-I conjugates are significantly brighter than fluorescein conjugates and much more photostable.

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DyLight™ Antibody Labeling, Long Stokes Shift Dyes, Thermo Scientific

DyLight™ Antibody Labeling, Long Stokes Shift Dyes, Thermo Scientific

Supplier: Invitrogen

The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 - 145 nm in excitation/emission wavelengths.

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Pierce™ EZ-Link™ Maleimide Activated Horseradish Peroxidase Kit, Thermo Scientific

Pierce™ EZ-Link™ Maleimide Activated Horseradish Peroxidase Kit, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce Maleimide Activated Horseradish Peroxidase is for preparation of HRP conjugates with proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

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EasyStain™ Immunofluorescence Reagent, BTF Precise Microbiology

Supplier: BIOPOINT USA INC MS

EasyStain™ is an immunofluorescence reagent designed for use in testing water samples for the presence of Cryptosporidium oocysts and Giardia cysts.

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PhosphoBlocker™ Blocking Reagent, Cell Biolabs

PhosphoBlocker™ Blocking Reagent, Cell Biolabs

Supplier: Cell Biolabs

PhosphoBLOCKER™ Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio

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AnaTag™ 5 - FITC Protein Labeling Kit *Ultra Convenient*, Anaspec

Supplier: Anaspec

Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivity to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels.

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Pierce EZ-Link® Carboxyl Reactive Biotinylation Reagents, Thermo Scientific

Pierce EZ-Link® Carboxyl Reactive Biotinylation Reagents, Thermo Scientific

Supplier: Invitrogen

Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatized biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4.5 - 5 and requires just minutes to complete.

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AnaTag™ 5 - TAMRA Protein Labeling Kit *Ultra Convenient*, Anaspec

Supplier: Anaspec

TAMRA is one of the most popular fluorophores used in various bioconjugations. 5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates.

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Pierce™ Deuterium-labeled Cross Linkers, Thermo Scientific

Pierce™ Deuterium-labeled Cross Linkers, Thermo Scientific

Supplier: Invitrogen

Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

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Pierce EZ-Link® Solid Phase Biotinylation Kits, Thermo Scientific

Pierce EZ-Link® Solid Phase Biotinylation Kits, Thermo Scientific

Supplier: Invitrogen

This antibody labeling system uses nickel chelated agarose to temporarily immobilize antibody molecules via their histidine-rich Fc regions

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Annexin V Binding Buffer

Annexin V Binding Buffer

Supplier: Stemcell Technologies

Assay buffer for labeling with Annexin V - 50 ml.

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Pierce™ iodoTMTzero™ Protein Labelling Reagent, 5×0.2 mg, Thermo Scientific

Pierce™ iodoTMTzero™ Protein Labelling Reagent, 5×0.2 mg, Thermo Scientific

Supplier: Invitrogen

The iodoTMTzero label reagent can be used as an isotopic 'light' duplex tag for MS-level protein quantitation.

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Pierce™ EZ-Link™ Activated Peroxidase Antibody Labeling Kit, Thermo Scientific

Pierce™ EZ-Link™ Activated Peroxidase Antibody Labeling Kit, Thermo Scientific

Supplier: Invitrogen

The Thermo Scientific Pierce Activated Peroxidase and Antibody Labeling Kit is designed to prepare and purify HRP-conjugated antibodies with any previously unmodified primary or secondary antibody (IgG).

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GloCell™ Fixable Viability Dye Violet 450

GloCell™ Fixable Viability Dye Violet 450

Supplier: Stemcell Technologies

Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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Pierce™ TMT10plex™ Isobaric Mass Tag Labelling Kit and Reagent Sets, Thermo Scientific

Pierce™ TMT10plex™ Isobaric Mass Tag Labelling Kit and Reagent Sets, Thermo Scientific

Supplier: Invitrogen

TMT10plex™ Isobaric Mass Tag Labeling Reagents Sets enable multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS).

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Pierce™ iodoTMTsixplex™ Isobaric Mass Tag Labeling Kit, Thermo Scientific

Pierce™ iodoTMTsixplex™ Isobaric Mass Tag Labeling Kit, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific iodoTMTsixplex Isobaric Mass Tag Labeling Kit contains iodoTMT™ reagents and associated materials for the irreversible labeling of sulfhydryl groups.

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StabilZyme® AP Conjugate stabilizer, Surmodics

Supplier: Surmodics

StabilZyme® AP and StabilZyme® Protein-Free AP stabilizers provide assay developers a complete set of tools to stabilize different AP conjugates for long term, in-solution stability.

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AnaTag™ HiLyte™ Fluor 488 Microscale Protein Labeling Kit, Anaspec

Supplier: Anaspec

HiLyte™ Fluor 488, SE is an excellent amine-reactive fluorescent labeling dye for generating protein conjugates. The spectrum of HiLyte™ Fluor488 is similar to fluorescein (FITC), resulting in an optimal match to filters designed for fluorescein.

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AnaTag™ HiLyte™ Fluor 555 Microscale Protein Labeling Kit *Ultra Convenient*, Anaspec

Supplier: Anaspec

HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy™ 3 dyes, resulting in an optimal match to filters designed for CyTM dyes.

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Pierce™  DyLight™ Fluor-Free Acids, Antibody Labelling Dyes, Thermo Scientific

Pierce™ DyLight™ Fluor-Free Acids, Antibody Labelling Dyes, Thermo Scientific

Supplier: Invitrogen

DyLight™ fluor-free acids are non-activated forms of selected DyLight™ fluorescent dyes for use as controls and calibration factors in fluorescence imaging applications

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Pierce™ NHS-Azide and NHS-Phosphine Reagents, Thermo Scientific

Pierce™ NHS-Azide and NHS-Phosphine Reagents, Thermo Scientific

Supplier: Invitrogen

The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.

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SpectraDye Antibody Labeling Kits, Advansta

Supplier: ADVANSTA INC MS

Make your own fluorescent antibody in one easy step.

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LysoBrite™ Lysosome Dye, Red DND-99

LysoBrite™ Lysosome Dye, Red DND-99

Supplier: Stemcell Technologies

Fluorescent dyes used to label and track lysosomes in live cells - 500 tests.

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Pierce™ iodoTMTsixplex™ Isobaric Label Reagent Set, Thermo Scientific

Pierce™ iodoTMTsixplex™ Isobaric Label Reagent Set, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific iodoTMTsixplex Label Reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry.

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Pierce™ Azide Labeled Sugars, Thermo Scientific

Pierce™ Azide Labeled Sugars, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce™ Azide-Labeled sugars provide a highly specific approach for studying glycoproteins through in vivo metabolic labeling and chemoselective ligation.

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