You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
AnaTag™ HiLyte™ Fluor 488 Protein Labeling Kit [3 labeling reactions (3×5 mg protein)], Anaspec
Supplier: Anaspec
HiLyte™ Fluor 488, SE is an excellent amine-reactive fluorescent labeling dye for generating protein conjugates. The spectrum of HiLyte™ Fluor488 is similar to fluorescein (FITC), resulting in an optimal match to filters designed for fluorescein.
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GloCell™ Fixable Viability Dye Violet 510
Supplier: Stemcell Technologies
Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.
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AnaTag™ HiLyte™ Fluor 555 Protein Labeling Kit *Ultra Convenient* [3 labeling reactions (3 x 5 mg protein)], Anaspec
Supplier: Anaspec
HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy™ 3 dyes, resulting in an optimal match to filters designed for CyTM dyes.
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LysoBrite™ Lysosome Dye, Green
Supplier: Stemcell Technologies
Fluorescent dyes used to label and track lysosomes in live cells - 500 tests.
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Pierce™ GlycoLink™ Coupling Catalyst, Thermo Scientific
Supplier: Invitrogen
An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.
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Biotin Conjugate Collagen Hybridizing Peptide (Biotin), Advanced Biomatrix
Supplier: Advanced Biomatrix
CHP can specifically bind to denatured collagen.
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5-FAM Conjugate Collagen Hybridizing Peptide (Green), Advanced Biomatrix
Supplier: Advanced Biomatrix
CHP can specifically bind to denatured collagen.
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Pierce™ CA(PEG)n Carboxy-PEG-Amine Compound, Thermo Scientific
Supplier: Invitrogen
The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers
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AnaTag™ HiLyte™ Fluor 750 Microscale Protein Labeling Kit *Ultra Convenient*, Anaspec
Supplier: Anaspec
HiLyte™ Fluor 750 acid, SE is the longest-wavelength amine-reactive HiLyte™ Fluor dye currently available. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores such as HiLyte™ Fluor 647, HiLyte™ Fluor 680 or allophycocyanin (APC), facilitating multicolor analysis.
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Pierce™ EZ-Link® Sulfhydryl Reactive Biotinylation Reagents, Thermo Scientific
Supplier: Invitrogen
Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.
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Pierce™ DyLight™ PEGylated Dyes, Antibody Labelling Dyes, Thermo Scientific
Supplier: Invitrogen
DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.
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GFP-Traps, Bulldog Bio
Supplier: Bulldog Bio
Novel Antibody Fragment that binds GFP Fusion Proteins.
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Pierce™ PEGylated SMCC Heterobifunctional Crosslinkers, Thermo Scientific
Supplier: Invitrogen
Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.
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Pierce™ Reduce-Imm™ Reducing Column, Thermo Scientific
Supplier: Invitrogen
Eliminate soluble components and reduce disulfide bonds without sacrificing valuable protein or peptide samples.
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StabilZyme® Protein-Free Stabilizer, Surmodics
Supplier: Surmodics
StabilZyme® HRP, StabilZyme® Protein-Free and StabilZyme® NOBLE stabilizers provide the assay developer a complete set of tools to stabilize different HRP conjugates for long term, in-solution stability.
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AnaTag™ AMCA - X Protein Labeling Kit *Ultra Convenient*, Anaspec
Supplier: Anaspec
AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer between the fluorophore and the reactive group.
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StabilZyme® Noble Stabilizer, Surmodics
Supplier: Surmodics
StabilZyme® HRP, StabilZyme® Protein-Free and StabilZyme® NOBLE stabilizers provide the assay developer a complete set of tools to stabilize different HRP conjugates for long term, in-solution stability.
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Pierce™ DyLight™ Antibody Labeling Kits, Thermo Scientific
Supplier: Invitrogen
The DyLight™ Antibody Labeling Kits and were specifically developed for fast, efficient labeling of antibodies
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GloCell™ Fixable Viability Dye UV 450
Supplier: Stemcell Technologies
Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.
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Pierce™ aminoxyTMTsixplex™ Label Reagent Set, Thermo Scientific
Supplier: Invitrogen
The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) label reagents enable multiplexed characterization and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidized proteins) by mass spectrometry (MS).
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Pierce™ Fluor Phosphine Reagents, DyLight™, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific DyLight Fluor Phosphine Reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.
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LysoBrite™ Lysosome Dye, Deep Red
Supplier: Stemcell Technologies
Fluorescent dyes used to label and track lysosomes in live cells - 500 tests.
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Pierce™ EZ-Link™ Plus Activated Peroxidase, Thermo Scientific
Supplier: Invitrogen
Activated peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins.
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Pierce™ DyLight™ Antibody Labeling, NHS Ester Quenchers, Thermo Scientific
Supplier: Invitrogen
DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes
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CY3 Conjugate Collagen Hybridizing Peptide (Red) , Advanced Biomatrix
Supplier: Advanced Biomatrix
CHP can specifically bind to denatured collagen.
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AnaTag™ 5 - FITC Microscale Protein Labeling Kit *Ultra Convenient*, Anaspec
Supplier: Anaspec
Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivity to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels.
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Pierce™ EZ-Link™ Amine Reactive Biotinylation Reagents, Thermo Scientific
Supplier: Invitrogen
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
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Pierce™ Pegylation Reagents, Methyl-PEG-Amine Compounds, Thermo Scientific
Supplier: Invitrogen
The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers
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BirA Biotin-Protein Ligase Reaction Kits, Avidity
Supplier: AVIDITY LLC MS
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner.
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Pierce™ ABC Peroxidase Standard Staining Kit, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.