You searched for: Antibodies

Supplier: Rockland Immunochemical

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Supplier: Rockland Immunochemical

6X His Tag CY3 conjugated Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.

Supplier: Immunoreagents

Igg (H&L), Affinity Pure, Polyclonal antibody, Host: LlamaSpecies: Mouse, Immunogen: Purified Mouse, IgG, whole molecule, Preservative: 0.05% (w/v) Sodium Azide, Applications: immunomicroscopy, flow cytometry

Supplier: Rockland Immunochemical

The guanylate-binding proteins (GBPs), a family of interferon-induced GTP-binding proteins function in innate immunity against microbial and viral pathogens. In humans, there are seven GBPs from hGBP1 to hGBP7. Human GBP1 and GBP2 have been shown to exhibit antiviral activity as well as being able to regulate the inhibition of proliferation and invasion of endothelial cells in response to interferon (IFN) (1,3). GBP6 is induced by IFN and hydrolyzes GTP to both GDP and GMP. The precise function of GBP6 is still under investigation.

Supplier: Rockland Immunochemical

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.

Supplier: Rockland Immunochemical

EMX1 is a homeobox transcription factor involved in specifying cell fates in the developing central nervous system and it participates in the development of olfactory neurons. EMX1 is specifically expressed in the developing telencephalic cortex expression, where epxression is restricted to pyramidal neurons. EMX1 is a reliable marker of pyramidal neurons and pyramidal cell lineage. EMX1 has been shown to be one of the downstream target genes for Gli-Kruppel family member 3 (Gli3) transcription factor, which is a part of the Sonic hedgehog-Patched-Gli signaling pathway important in endocrine signaling.

Supplier: Rockland Immunochemical

F(ab')2 Mouse IgG (H&L), Polyclonal antibody, Host: Goat, Target Species: Mouse, Format: IgG F(ab')2, Label: Fluorescein (FITC), Immunogen: Mouse IgG whole molecule, Synonyms: FITC, Applications: immunomicroscopy, flow cytometry, FACS analysis, antibody based fluorescent assays, Size: 20mg

Supplier: Rockland Immunochemical

F(ab')2 Sheep IgG (H&L), Polyclonal antibody, Host: Rabbit, Target Species: Sheep, Format: IgG F(ab')2, Label: Fluorescein (FITC), Immunogen: Sheep IgG whole molecule, Synonyms: FITC, Application: immunomicroscopy, flow cytometry, FACS analysis, antibody based fluorescent assay, Size: 20mg

Supplier: Rockland Immunochemical

EMX2, like its closely related homolog EMX1, is a homeobox transcription factor involved in specifying cell fates in the developing central nervous system and participates in the development of olfactory neurons. EMX2 is expressed in the dorsal telencephalon during development in a low rostral-lateral to high caudal-medial gradient and is proposed to pattern the neocortex into defined functional areas. It is also expressed in embryonic and adult olfactory neuroepithelia where it complexes with eukaryotic translation initiation factor 4E (eIF4E) and possibly regulates mRNA transport or translation. In the developing urogenital system, it is expressed in epithelial tissues and is negatively regulated by HOXA10.

Supplier: Rockland Immunochemical

Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. Of the CTRPs, CTRP2 is most similar structurally and functionally to adiponectin. Recombinant CTRP2 rapidly activated AMPK and MAPK in cultured C2C12 cells, leading to increased glycogen accumulation and fatty acid oxidation.

Supplier: Rockland Immunochemical

CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, murine, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines. Anti-CX3CR1 is ideal for investigators involved in cytokines, growth factors and infectious diease research.

Supplier: Rockland Immunochemical

Anti-Human Igg (Handl) (Donkey) Antibody Fluorescein Conjugated - 609-702-002 Human Igg (Handl) Antibody Fluorescein Conjugated 2 Mg

Supplier: Rockland Immunochemical

Anti-Rat Igg (Handl) (Donkey) Antibody Rhodamine Conjugated - 612-700-002 Rat Igg (Handl) Antibody Rhodamine Conjugated 2 Mg

Supplier: Rockland Immunochemical

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Supplier: Rockland Immunochemical

The CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) domain is an evolutionarily conserved, ~40 amino acid monoubiquitin-binding domain that mediates intramolecular monoubiquitylation. CUE domains are present in eukaryotic proteins that are involved in ubiquitination and protein trafficking pathways and may be required for ubiquitination of the proteins in which they are found. CUEDC2 (CUE domain-containing protein 2) was found through a yeast two-hybrid screening as a protein that interacts with the progesterone receptor (PR) and promotes progesterone-induced PR degradation by the ubiquitin-proteasome pathway. CUEDC2 also decreases the sumoylation of PR. CUEDC2 has also been found to interact with IKK-alpha and IKK-beta and decrease the activation of NF-kB by decreasing the activation of IKK.

Supplier: Rockland Immunochemical

The Breast carcinoma amplified sequence (BCAS3) gene is localized to 17q23, a region that is often amplified in breast cancers. Copy number gains at this region have also been reported in other tumors such as brain, lung, liver, testis and bladder. BCAS3 is thought to be an estrogen receptor (ER)-alpha co-activator that acts through PELP1, another ER-alpha that in turn activates BCAS3 expression. This may set up a positive feedback loop leading to ER-a signal amplification in the cell which may play a significant role in breast cancer. BCAS3 expression has also been observed in embryonic stem cells and vascular precursors, suggesting that it may also play a role in embryogenesis and tumor angiogenesis.

Supplier: Rockland Immunochemical

Suitable for western blotting, immunochemistry and other antibody based assays. A 1:500 dilution is recommended for western blotting.

Supplier: Rockland Immunochemical

Anti-Penicillinase (Enterobacter Cloacae) (Rabbit) Antibody Biotin Conjugated - Anti-Penicillinase Antibody Has Been Assayed Against 1 Ug Of Penicillinase In A Standard Capture ELISA Using Peroxidase Conjugated Streptavidin.

Supplier: Rockland Immunochemical

This Protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 30.3 kDa in size corresponding to Borrelia burgdorferi OspB Protein by western blotting.

Supplier: Rockland Immunochemical

Anti-Retinoic Acid Receptor alpha antibody is suitable for use in Western Blotting to detect a single band at approximately 48 kDa corresponding to expected molecualr weight for RAR-a Proteins in the appropriate cell lysate or extract.

Supplier: Rockland Immunochemical

A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Mouse IgM antibody is suitable for immunosassays including western blot and ELISA.

Supplier: Rockland Immunochemical

Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. ARC interacts with caspase-2 and -8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.

Supplier: Rockland Immunochemical

This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 28 kDa or 26 kDa in size corresponding to variants of ING5 by wester blotting.

Supplier: Immunoreagents

HRP Affinity Pure, Polyclonal antibody, Host: Goat, Preservative: 0.05% (w/v) Sodium Azide, Concentration: > 4.5 mg/ml (E 1% at 280 nm = 13.0), Purity: > 95% based on SDS-PAGE, Applications: Immunoassay

Supplier: Rockland Immunochemical

The Anti-Pax7 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Paired box protein Pax-7 transcription factor plays a role in myogenesis through regulation of muscle precursor cells proliferation. These genes play critical roles during fetal development and cancer growth. It is expressed specifically during the development of the nervous and muscular system. Anti-Pax7 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Supplier: Rockland Immunochemical

CAT IgG F(ab')2 (RABBIT) Antibody Peroxidase Conjugated, Polyclonal antibody, Host: Rabbit, Label: HRP, Immunogen: Cat IgG F(ab')2 fragment, Storage: Store vial at 4C prior to restoration, Size: 20MG

Supplier: Rockland Immunochemical

Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF). PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR) and capillary morphogenesis protein 2 (CMG2), although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm. These toxins are usually sufficient to cause rapid cell death, and often the death of the organism.

Supplier: Rockland Immunochemical

Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK BCL10, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. ARC interacts with caspase-2 and -8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1, and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.

Supplier: Rockland Immunochemical

Rabbit Anti-VhH antibody can be used to detect single domain VhH antibodies. The single-domain antibody (sdAb) is a small (12 - 14 kDa) antibody fragment that consists of a monomeric variable domain derived from the heavy chain, also called a VhH antibody. These heavy chain only Fab-like domains have activity that is similar to a whole antibody, and they are able to bind to a specific antigens. SdAb's are derived from camelid species that include llamas, alpacas and camels. Camelids produce both classical (containing heavy and light chain fragments) and non-classical antibody structures (containing only a heavy chain). VhH antibodies are the smallest functional antigen-binding fragment that occurs in nature and these are now being used in biotechnology as a novel antibody scaffold. The small size of the VhH single domain antibody makes it very attractive for use in diagnostic imaging and they have potential for therapeutic activity.

Supplier: Rockland Immunochemical

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Aven is a recently discovered protein that blocks apoptosis induced by Apaf-1 and caspase-9. It is thought that Aven functions by binding to Bcl-xL, an antiapoptotic member of the Bcl-2 family, and to Apaf-1, possibly interfering with the ability of Apaf-1 to self-associate, suggesting that Aven impedes Apaf-1-mediated caspase activation. Higher levels of Aven mRNA are seen in patients with acute leukemia than in control patients, suggesting that Aven may be useful as a prognostic indicator in leukemia patients.