You searched for: End-point PCR Enzymes and Kits
Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.
Q5U Hot Start High-Fidelity DNA Polymerase, New England Biolabs
Supplier: New England Biolabs (NEB)
Q5U Hot Start high-fidelity DNA Polymerase is a modified version of Q5 high-fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3' to 5' exonuclease activity, and is fused to a processivity-enhancing Sso7d domain.
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GoTaq® G2 Green Master Mix, 1000 reactions, Promega
Supplier: Promega Corporation
GoTaq(R) G2 Master Mixes are premixed ready-to-use solutions containing GoTaq(R) G2 DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of a wide range of DNA templates by PCR.
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GoTaq Green Master Mix, 1,000 reactions, Promega
Supplier: Promega Corporation
New formulation of Taq DNA Polymerase with enhanced performance over standard Taq DNA Polymerase. Available in two all-in-one master mixes: green and colorless. The green master mix allows direct gel analysis after amplification.
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RNase 4 Digestion and 3' End Repair Mix
Supplier: New England Biolabs (NEB)
RNase 4 is a single-stranded RNA endonuclease that cleaves 3′ of uridine in uridine-purine sequences (cut sites: U/A and U/G).
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GoTaq Hot Start Green Master Mix, Promega
Supplier: Promega Corporation
GoTaq Hot Start Master Mixes are all-in-one master mixes (just add template and primers) that contain the high-performance GoTaq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 94-95°C for two
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GoTaq® Colorless Master Mix, 1000 reactions, Promega
Supplier: Promega Corporation
New formulation of Taq DNA Polymerase with enhanced performance over standard Taq DNA Polymerase. Available in two all-in-one master mixes: green and colorless. The green master mix allows direct gel analysis after amplification.
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ONETAQ® RT-PCR Kit, New England Biolabs
Supplier: New England Biolabs (NEB)
OneTaq® RT-PCR Kit combines two powerful mixes, M-MuLV Enzyme Mix and OneTaq Hot Start 2X Master Mix with Standard Buffer for 2-step RT-PCR applications.
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GoTaq® Reaction Buffers for PCR, Promega
Supplier: Promega Corporation
Available with green dye for direct-to-gel convenience, or in colorless format for applications where absorbance or fluorescence measurements are necessary prior to PCR cleanup
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MangoTaq™ DNA Polymerase, Bioline
Supplier: MERIDIAN LIFE SCICNE, INC BE
MangoTaq™ DNA Polymerase is a formulation of Taq DNA Polymerase that offers high-yield across a wide range of DNA concentrations.
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MeanGreen™ Taq 2X MasterMix
Supplier: EMPIRICAL BIOSCIENCE
MasterMix supplied as a 2X reaction buffer with Loading Dye, dCTP, dGTP, dATP, dTTP, MgCl2 and Taq DNA polymerase.
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VENT® (exo-) DNA Polymerase, New England Biolabs
Supplier: New England Biolabs (NEB)
VentR (exo-) DNA Polymerase has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity associated with VentR DNA Polymerase
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Bst DNA Polymerase Lg Frag, New England Biolabs
Supplier: New England Biolabs (NEB)
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity.
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Phusion High-Fidelity PCR Master Mix with GC Buffer, New England Biolabs
Supplier: New England Biolabs (NEB)
Phusion High-Fidelity PCR Master Mix with GC Buffer offers robust performance and can be used for all PCR applications
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LONGAMP® Hot Start Taq DNA Polymerase, New England Biolabs
Supplier: New England Biolabs (NEB)
LongAmp Hot Start Taq DNA Polymerase is a unique blend of aptamer-based Hot Start Taq and Deep VentR™ DNA Polymerases.
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Q5® Hot Start High-Fidelity 2X Master Mix
Supplier: New England Biolabs (NEB)
The Q5 Hot start high-fidelity 2X master mix features a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.
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FASTaq™ HotStart DNA Polymerase
Supplier: EMPIRICAL BIOSCIENCE
Chemically modified Taq DNA polymerase designed for reducing non-specific DNA amplification due to primer-dimer formation. FASTaq™ is completely suppressed prior to activation, and regains full enzymatic activity after 10 min at 95 °C. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli.
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PHU-50 High Fidelity Polymerase
Supplier: EMPIRICAL BIOSCIENCE
Phu-50 High Fidelity Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Phu DNA polymerase but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5 - 3' direction but does not possess a 5 - 3' exonuclease replacement activity. Its inherent 3 - 5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Phu-50 Polymerase-generated PCR fragments are blunt.
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EPIMARK® Hot Start Taq DNA Polymerase, New England Biolabs
Supplier: New England Biolabs (NEB)
EpiMark Hot Start Taq DNA Polymerase an excellent choice for use on bisulfite-converted DNA
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Taq 2X Master Mix, New England Biolabs
Supplier: New England Biolabs (NEB)
Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers
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DEEP VENT™ (exo-) DNA Polymerase, New England Biolabs
Supplier: New England Biolabs (NEB)
Deep VentR (exo-) DNA Polymerase has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity associated with Deep Vent DNA Polymerase
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Bst 2.0 DNA Polymerase, New England Biolabs
Supplier: New England Biolabs (NEB)
Bst 2.0 DNA Polymerase is an in silico designed homologue of Bacillus stearo thermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment)
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GeneMate Taq DNA Polymerase
Supplier: GeneMate
Taq DNA Polymerase is a thermostable DNA polymerase, which can withstand prolonged incubation at high temperatures without a significant loss of activity
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ONETAQ® Hot Start DNA Polymerase, New England Biolabs
Supplier: New England Biolabs (NEB)
OneTaq® Hot Start DNA Polymerase is an optimized blend of Taq and Deep VentR™ DNA polymerases combined with an aptamer-based inhibitor.
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Hot Start Taq 2X Master Mix, New England Biolabs
Supplier: New England Biolabs (NEB)
Hot Start Taq 2X Master Mix is an optimized ready-to-use solution containing Hot Start Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers
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LONGAMP® Hot Start Taq 2X Master Mix, New England Biolabs
Supplier: New England Biolabs (NEB)
LongAmp Hot Start Taq 2X Master Mix contains a unique blend of aptamer-based Hot Start Taq and Deep VentR™ DNA Polymerases.
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Phusion Hot Start Flex 2X Master Mix, New England Biolabs
Supplier: New England Biolabs (NEB)
Phusion Hot Start Flex 2X Master Mix offers robust high fidelity performance and room temperature reaction setup in a convenient master mix format
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ONETAQ® Hot Start 2X Master Mix with GC Buffer, New England Biolabs
Supplier: New England Biolabs (NEB)
OneTaq® Hot Start 2X Master Mix with GC Buffer is an optimized blend of Taq and Deep VentR ™ DNA Polymerases combined with an aptamer-based inhibitor.
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High Fidelity DNA Polymerase
Supplier: G-Biosciences
High-fidelity DNA Polymerase possesses 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This Polymerase has the fidelity, sensitivity and processivity with an error rate ~2.8×10² fold lower than Taq DNA polymerase, and significantly lower than other proofreading DNA polymerases.
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Q5® Hot Start High-Fidelity DNA Polymerase
Supplier: New England Biolabs (NEB)
Q5 Hot Start High-Fidelity DNA Polymerase is a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.
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GoTaq® G2 Hot Start Green Master Mix, Promega
Supplier: Promega Corporation
GoTaq G2 Hot Start Master Mixes are ready-to-use mixes containing GoTaq G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium