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250 results for Vectors, Plasmids and Libraries

You searched for: Vectors, Plasmids and Libraries

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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pGloSensor-22F cAMP Plasmid, 20 µg, Promega

Supplier: Promega Corporation

The GloSensor cAMP Assay is based on a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.

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pFN2A (GST) Flexi Vector, 20 µg, Promega

pFN2A (GST) Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

These vectors are used for inducible expression of N-terminal GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

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pAdVAntage Vector, 20 µg, Promega

pAdVAntage Vector, 20 µg, Promega

Supplier: Promega Corporation

Co-transfection of mammalian cells with the pAdVAntage Vector enhances transient protein expression in a variety of cell types by increasing translation initiation.

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pGL4.41[luc2P/HSE/Hygro] Vector, 20 µg, Promega

pGL4.41[luc2P/HSE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

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pGL4.15[luc2P/Hygro] Vector, 20 µg, Promega

pGL4.15[luc2P/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.14[luc2/Hygro], pGL4.15[luc2P/Hygro] and pGL4.16[luc2CP/Hygro] Vectors have different luc2 genes that code for luciferase proteins with different stabilities. Each vector offers hygromycin for stable selection.

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pMAL-c6T Vector, New England Biolabs

pMAL-c6T Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

The pMAL-c6T Vector is designed to produce maltose-binding protein (MBP) fusions in the cytoplasm.

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pFN31K Nluc CMV-neo Flexi Vector, 20 µg, Promega

pFN31K Nluc CMV-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.

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pFN21A HaloTag CMV Flexi Vector, 20 µg, Promega

pFN21A HaloTag CMV Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

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VSV-Pseudovirus_Ebola Bomali Strain Luciferase

VSV-Pseudovirus_Ebola Bomali Strain Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Bombali Ebolavirus (GenBank: YP_009513277.1).

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West Nile Virus (WNV) Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

West Nile Virus (WNV) Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

Supplier: ReVacc Scientific

Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of West Nile Virus (WNV) (GenBank: DQ211652.1, NY99 strain) and a Renilla luciferase reporter. As the wild typed WNV is handled under BSL-2 precautions, this WNV RVP can be handled using BSL-2 containment practices.

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pGL4.25[luc2CP/minP] Vector, 20 µg, Promega

pGL4.25[luc2CP/minP] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.23[luc2/minP], pGL4.24[luc2P/minP] and pGL4.25[luc2CP/minP] Vectors offer different firefly luciferase protein stabilities for expression in mammalian cells. Clone a response element of interest upstream of the minimal promoter and reporter gene.

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VSV-Pseudovirus_Zaire Ebola Luciferase, ReVacc Scientific

VSV-Pseudovirus_Zaire Ebola Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Zaire ebolavirus Makona strain (GenBank: KR781609.1).

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pGL4.37[luc2P/ARE/Hygro] Vector, 20 µg, Promega

pGL4.37[luc2P/ARE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

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M13K07 Helper Phage, New England Biolabs

M13K07 Helper Phage, New England Biolabs

Supplier: New England Biolabs (NEB)

M13KO7 is an M13 derivative which carries the mutation Met40Ile in gII, with the origin of replication from P15A and the kanamycin resistance gene from Tn903 both inserted within the M13 origin of replication

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pGL4.36[luc2P/MMTV/Hygro] Vector, 20 µg, Promega

pGL4.36[luc2P/MMTV/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.

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pGL4.38[luc2P/p53 RE/Hygro] Vector, 20 µg, Promega

pGL4.38[luc2P/p53 RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

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pGEM-7Zf(+) Vector, 20 µg, Promega

pGEM-7Zf(+) Vector, 20 µg, Promega

Supplier: Promega Corporation

Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.

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pGL4.50[luc2/CMV/Hygro] Vector, 20 µg, Promega

pGL4.50[luc2/CMV/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4 Luciferase Reporter Vectors are the next generation of luciferase reporter vectors optimized for expression in mammalian cells.

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pNL1.1.TK[Nluc/TK] Vector, 20 µg, Promega

pNL1.1.TK[Nluc/TK] Vector, 20 µg, Promega

Supplier: Promega Corporation

The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System.

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pNL2.2[NlucP/Hygro] Vector, 20 µg, Promega

pNL2.2[NlucP/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. pNL2.1[Nluc/Hygro] and pNL2.2[NlucP/Hygro] Vectors are used for cloning putative promoters; select for stable cell lines using hygromycin.

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pHTN HaloTag CMV-neo Vector, 20 µg, Promega

pHTN HaloTag CMV-neo Vector, 20 µg, Promega

Supplier: Promega Corporation

Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

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pNL1.2[NlucP] Vector, 20 µg, Promega

pNL1.2[NlucP] Vector, 20 µg, Promega

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL1.1[Nluc] and pNL1.2[NlucP] Vectors are used to clone putative promoter regions to express the bright NanoLuc(R) luciferase.

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pFC30K His6HaloTag T7 Flexi Vector, 20 µg, Promega

pFC30K His6HaloTag T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.

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pSP73 Vector, 20 µg, Promega

pSP73 Vector, 20 µg, Promega

Supplier: Promega Corporation

Used as standard cloning vector or for transcription of RNA in vitro.

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VSV-Pseudovirus_SARS-CoV-2 Beta Luciferase, ReVacc Scientific

VSV-Pseudovirus_SARS-CoV-2 Beta Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry spike protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations identified in Beta variant: L18F, D80A, D215G, L242-244del, R246I, K417N, E484K, N501Y, D614G, and A701V. The spike protein has a 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_SARS-CoV Luciferase, ReVacc Scientific

VSV-Pseudovirus_SARS-CoV Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry S protein of SARS-CoV (GenBank: AY278554.2) (2003 strain), with 28-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_Marburg Ravn Luciferase, ReVacc Scientific

VSV-Pseudovirus_Marburg Ravn Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Ravn (GenBank: DQ447649.1).

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pGL4.44[luc2P/AP1 RE/Hygro] Vector, 20 µg, Promega

pGL4.44[luc2P/AP1 RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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pGL4.16[luc2CP/Hygro] Vector, 20 µg, Promega

pGL4.16[luc2CP/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.14[luc2/Hygro], pGL4.15[luc2P/Hygro] and pGL4.16[luc2CP/Hygro] Vectors have different luc2 genes that code for luciferase proteins with different stabilities. Each vector offers hygromycin for stable selection.

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pGEM-5Zf(+) Vector, 20 µg, Promega

pGEM-5Zf(+) Vector, 20 µg, Promega

Supplier: Promega Corporation

Used as a standard cloning vector and as a template for in vitro transcription.

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