You searched for: Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
pBR322 Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pBR322 DNA is a commonly used plasmid cloning vector in E.coli.
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pFN28K HaloTag CMV-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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pFN28A HaloTag CMV-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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pGL4.42[luc2P/HRE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.
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pF3K WG (BYDV) Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest.
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pF4K CMV Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Designed for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker
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VSV-Pseudovirus_SARS-COV-2 Omicron XBB.1.16 Luciferase
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron XBB.1.16. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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pGL4.44[luc2P/AP1 RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.
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pGL4.16[luc2CP/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.14[luc2/Hygro], pGL4.15[luc2P/Hygro] and pGL4.16[luc2CP/Hygro] Vectors have different luc2 genes that code for luciferase proteins with different stabilities. Each vector offers hygromycin for stable selection.
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pGEM-5Zf(+) Vector, 20 µg, Promega
Supplier: Promega Corporation
Used as a standard cloning vector and as a template for in vitro transcription.
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pGL4.37[luc2P/ARE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.
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pFC14K HaloTag CMV Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of C-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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Dengue Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter (Type 1)
Supplier: ReVacc Scientific
Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of Dengue virus (DENV) (GenBank: AAB70694.1, SEROTYPE 1 strain) and a Renilla luciferase reporter. As the wild typed Dengue virus is handled under BSL-2 precautions, this Dengue virus RVP can be handled using BSL-2 containment practices.
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VSV-Pseudovirus_HCV H77 Strain Luciferase
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the full length E1E2 of Hepatitis C virus (HCV) (H77 strain) (GenBank: AAB67036.1).
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Ph.D.-C7C™ Phage Display Peptide Library Kit v2
Supplier: New England Biolabs (NEB)
The Ph.D.-C7C Phage display peptide library kit v2 is based on a combinatorial library of random disulfide looped peptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage.
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pGL4.21[luc2P/Puro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.20[luc2/Puro], pGL4.21[luc2P/Puro] and pGL4.22[luc2CP/Puro] Vectors are optimized for expression in mammalian cells. The luc2 genes code for luciferase proteins with different stabilities, and each vector offers puromycin for stable selection.
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M13K07 Helper Phage, New England Biolabs
Supplier: New England Biolabs (NEB)
M13KO7 is an M13 derivative which carries the mutation Met40Ile in gII, with the origin of replication from P15A and the kanamycin resistance gene from Tn903 both inserted within the M13 origin of replication
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pGL4.25[luc2CP/minP] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.23[luc2/minP], pGL4.24[luc2P/minP] and pGL4.25[luc2CP/minP] Vectors offer different firefly luciferase protein stabilities for expression in mammalian cells. Clone a response element of interest upstream of the minimal promoter and reporter gene.
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pGEM-7Zf(+) Vector, 20 µg, Promega
Supplier: Promega Corporation
Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.
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pNL2.2[NlucP/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. pNL2.1[Nluc/Hygro] and pNL2.2[NlucP/Hygro] Vectors are used for cloning putative promoters; select for stable cell lines using hygromycin.
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pNL1.1.TK[Nluc/TK] Vector, 20 µg, Promega
Supplier: Promega Corporation
The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System.
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VSV-Pseudovirus_Zaire Ebola Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Zaire ebolavirus Makona strain (GenBank: KR781609.1).
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pSP73 Vector, 20 µg, Promega
Supplier: Promega Corporation
Used as standard cloning vector or for transcription of RNA in vitro.
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pNL1.2[NlucP] Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL1.1[Nluc] and pNL1.2[NlucP] Vectors are used to clone putative promoter regions to express the bright NanoLuc(R) luciferase.
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pFC30K His6HaloTag T7 Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
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pHTN HaloTag CMV-neo Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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pGL4.38[luc2P/p53 RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.
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pGL4.36[luc2P/MMTV/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
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pGL4.50[luc2/CMV/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4 Luciferase Reporter Vectors are the next generation of luciferase reporter vectors optimized for expression in mammalian cells.
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VSV-Pseudovirus_SARS-CoV-2 Beta Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry spike protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations identified in Beta variant: L18F, D80A, D215G, L242-244del, R246I, K417N, E484K, N501Y, D614G, and A701V. The spike protein has a 18-aa cytoplasmic tail truncation for optimal infection.