You searched for: Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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VSV-Pseudovirus_SARS-COV-2 Omicron JN.1 Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron JN.1. See mutation on our website. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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M13mp18 RF I DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13.

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pCLIPf Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pCLIPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of CLIP-tag® protein fusions in mammalian cells

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pNL3.3[secNluc/minP] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

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pUC19 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pUC19 is a commonly used plasmid cloning vector in E.coli

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Zika Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

Supplier: ReVacc Scientific

Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of ZIKV (GenBank: KU321639.1, SPH2015 strain) and a Renilla luciferase reporter.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BF.7 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BF.7. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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pGL4.30[luc2P/NFAT-RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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pGloSensor-20F cAMP Plasmid, 20 µg, Promega

Supplier: Promega Corporation

The GloSensor cAMP Assay is based on a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.

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pF9A CMV hRluc-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

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pTNT Vector, 20 µg, Promega

Supplier: Promega Corporation

The pCMVTNT and pTNT Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems.

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VSV-Pseudovirus_Lassa Virus Josiah GPC Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry glycoprotein complex (GPC) of Lassa virus (LASV) Josiah Strain (GenBank: P08669).

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pF5K CMV-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

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pSNAP-TAG® (T7)-2 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein

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pHTC HaloTag CMV-neo Vector, 20 µg, Promega

Supplier: Promega Corporation

Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

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pSP72 Vector, 20 µg, Promega

Supplier: Promega Corporation

Used as standard cloning vector or for transcription of RNA in vitro.

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pNL1.3.CMV[secNluc/CMV] Vector, 20 µg, Promega

Supplier: Promega Corporation

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pGL4.29[luc2P/CRE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

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pTXB1 Vector DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

pTXB1 is an E.coli expression vector in the IMPACT™ Kit.

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pGL4.31[luc2P/GAL4UAS/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.

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pGL4.28[luc2CP/minP/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.26[luc2/minP/Hygro], pGL4.27[luc2P/minP/Hygro] and pGL4.28[luc2CP/minP/Hygro] Vectors are used to clone a response element of interest upstream of the minimal promoter and firefly luciferase gene with and without destabilization sequences.

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pGL4.53[luc2/PGK] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.13[luc2/SV40] ,pGL4.53[luc2/PGK] and pGL4.54[luc2/TK] Vectors are used as controls in dual-luciferase assays where Renilla or NanoLuc luciferase is the experimental reporter. Select the appropriate promoter or test all 3 in your experimental system.

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VSV-Pseudovirus_SARS-CoV-2 Alpha Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry spike protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations identified in variant of B.1.1.7 (Alpha strain), including: ΔH69/V70, ΔY144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H. The spike protein has a 18-aa cytoplasmic tail truncation for optimal infection

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VSV-Pseudovirus_Marburg Angola Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Angola (GenBank: KU978782.1).

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pF4K CMV Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Designed for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker

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pFN28A HaloTag CMV-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

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pF3K WG (BYDV) Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest.

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pFN28K HaloTag CMV-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

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pGL4.40[luc2P/MRE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.