You searched for: Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
pFC32K Nluc CMV-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.
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pNL1.1[Nluc] Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL1.1[Nluc] and pNL1.2[NlucP] Vectors are used to clone putative promoter regions to express the bright NanoLuc(R) luciferase.
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CheckMate Positive Control Vectors, 1 set, Promega
Supplier: Promega Corporation
Powerful system for detecting protein:protein interactions in vivo.
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pGEM-3Zf(+) Vector, 20 µg, Promega
Supplier: Promega Corporation
Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.
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pGEM-4Z Vector, 20 µg, Promega
Supplier: Promega Corporation
Intended for use as a standard cloning vector or for the high-fidelity efficient synthesis of RNA in vitro.
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HaloTag Control Vector, 20microg, Promega
Supplier: Promega Corporation
The HaloTag Mammalian Pull-Down Systems capture and purify intracellular protein complexes for correlative cellular localization and real-time imaging studies with the same genetic construct for further understanding of overall protein function.
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pFC14A HaloTag CMV Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of C-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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Ph.D.-12 Phage Display Peptide Library Kit v2, New England Biolabs
Supplier: New England Biolabs (NEB)
The Ph.D.−12 Phage display peptide library kit v2 is based on a combinatorial library of random dodecapeptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The peptide is followed by a short spacer (Gly-Gly-Gly) which directly precedes the wild-type pIII sequence. The library consists of ~1E09 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.
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PH.D.-7 Phage Display Peptide Library Kit v2, New England Biolabs
Supplier: New England Biolabs (NEB)
The Ph.D.−7 phage display peptide library kit v2 is based on a combinatorial library of random heptapeptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The peptide is followed by a short spacer (Gly-Gly-Gly) which directly precedes the wild-type pIII sequence. The library consists of ~1E09 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.
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pGL4.52[luc2P/STAT5RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.
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pFC27K HaloTag CMV-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of C-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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pFN26A (BIND) hRluc-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
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pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
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pGL4.34[luc2P/SRF-RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.
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CheckMate/Flexi Vector Mammalian Two-Hybrid System, 1 each, Promega
Supplier: Promega Corporation
The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.
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pGL4.51[luc2/CMV/Neo] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4 Luciferase Reporter Vectors are the next generation of luciferase reporter vectors optimized for expression in mammalian cells.
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pCI-neo Mammalian Expression Vector, 20 µg, Promega
Supplier: Promega Corporation
Contains a neomycin resistance gene and the CMV immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. Transfected cells can be selected with G-418.
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pGEM-3Z Vector, 20 µg, Promega
Supplier: Promega Corporation
Intended for use as a standard cloning vector or for high-fidelity efficient synthesis of RNA in vitro.
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VSV-Pseudovirus_SARS-CoV-2 Omicron XBB.1.5 Strain Spike with Luciferase Reporter
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron XBB.1.5. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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VSV-Pseudovirus_SARS-CoV-2 Omicron BQ.1.1 Strain Spike with Luciferase Reporter
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BQ.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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Lentivirus Pseudotyped Influenza A HPAI H5N1 virus HaNaM with Luciferase Reporter (clade 2.3.4.4b, 2022 strain)
Supplier: ReVacc Scientific
This pseudotyped virus uses lentivirus packaging to carry full length HA, NA and M of Highly Pathogenic Avian Influenza (HPAI) A virus (A/Pelecanus/Peru/VFAR-140/2022(H5N1), clade 2.3.4.4b) (GenBank: OQ565628.1). The pseudotypes can be handled using BSL-2 containment practices.
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pGEM® T Vector Systems, Promega
Supplier: Promega Corporation
The pGEM-T Vector Systems are convenient systems for cloning PCR products.
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VSV-Pseudovirus_SARS-COV-2 Omicron CH.1.1 Luciferase
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron CH.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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pRL-CMV Vector, 20 µg, Promega
Supplier: Promega Corporation
The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.
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pGL4.47[luc2P/SIE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.
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pLIVE® In Vivo Expression and Reporter Vector Kits, Mirus Bio
Supplier: Mirus Bio
pLIVE® vector is designed for high level, prolonged expression of transgenes in the mouse liver.
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VSV-Pseudovirus_MERS-CoV Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry S protein of MERS-CoV (GenBank: JX869059.2), with 16-aa cytoplasmic tail truncation for optimal infection.
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CheckMate Negative Control Vectors, 1 set, Promega
Supplier: Promega Corporation
Powerful system for detecting protein:protein interactions in vivo.
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pTYB21 Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pTYB21 is an E
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PhiX174 Virion DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70