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250 results for Vectors, Plasmids and Libraries

You searched for: Vectors, Plasmids and Libraries

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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pBR322 Vector, 10 µg, Promega

pBR322 Vector, 10 µg, Promega

Supplier: Promega Corporation

pBR322 Vector.

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pGL4.26[luc2/minP/Hygro] Vector, 20 µg, Promega

pGL4.26[luc2/minP/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.26[luc2/minP/Hygro], pGL4.27[luc2P/minP/Hygro] and pGL4.28[luc2CP/minP/Hygro] Vectors are used to clone a response element of interest upstream of the minimal promoter and firefly luciferase gene with and without destabilization sequences.

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pGL4.10[luc2] Vector, 20 µg, Promega

pGL4.10[luc2] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.10[luc2], pGL4.11[luc2P] and pGL4.12[luc2CP] Vectors are promoterless reporter vectors optimized for expression in mammalian cells. The different luc2 genes code for luciferase proteins with different stabilities.

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pF25A ICE T7 Flexi Vector, 20 µg, Promega

pF25A ICE T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency.

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VSV-Pseudovirus_SARS-CoV-2 Delta Luciferase, ReVacc Scientific

VSV-Pseudovirus_SARS-CoV-2 Delta Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of B.1.617.2 (Delta strain), and 18-aa cytoplasmic tail truncation for optimal infection.

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M13mp18 Single-Stranded DNA, New England Biolabs

M13mp18 Single-Stranded DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

The single-stranded viral DNA is isolated from M13mp18

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pH6HTN His6HaloTag T7 Vector, 20 µg, Promega

pH6HTN His6HaloTag T7 Vector, 20 µg, Promega

Supplier: Promega Corporation

These multiple cloning site vectors are used for inducible expression of HaloTag-polyhistidine fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

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pSNAPf Vector, New England Biolabs

pSNAPf Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pSNAPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of SNAP-tag® protein fusions in mammalian cells

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pFN29A His6HaloTag T7 Flexi Vector, 20 µg, Promega

pFN29A His6HaloTag T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.

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pGL4.48[luc2P/SBE/Hygro] Vector, 20 µg, Promega

pGL4.48[luc2P/SBE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.

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pGL4.18[luc2P/Neo] Vector, 20 µg, Promega

pGL4.18[luc2P/Neo] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.17[luc2/Neo] Vector, pGL4.18[luc2P/Neo] and pGL4.19[luc2CP/Neo] Vectors are optimized for expression in mammalian cells. These vectors offer standard and destablized luciferase with neomycin for stable selection.

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pNLF1-HIF1A [CMV/neo] Vector, 1 each, Promega

pNLF1-HIF1A [CMV/neo] Vector, 1 each, Promega

Supplier: Promega Corporation

NanoLuc Stability Sensors are ready-to-use vector systems that utilize the advantages of the NanoLuc luciferase reporter for stability studies of two key signaling proteins, HIF1A and NRF2, providing a way to directly measure the primary signaling event.

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pF1A T7 Flexi Vector, 20 µg, Promega

pF1A T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

These untagged vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Supplied as vectors used with the Flexi Cloning System.

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pFN31A Nluc CMV-Hygro Flexi Vector, 20 µg, Promega

pFN31A Nluc CMV-Hygro Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.

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VSV-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific

VSV-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) (Wuhan strain or Wild type strain), with 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_Marburg Musoke Luciferase, ReVacc Scientific

VSV-Pseudovirus_Marburg Musoke Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Musoke (GenBank: DQ217792.1).

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pF25K ICE T7 Flexi Vector, 20 µg, Promega

pF25K ICE T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.86 Luciferase

VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.86 Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudotyped HMPV Fusion with Luciferase Reporter (NL/1/00/A1 strain)

VSV-Pseudotyped HMPV Fusion with Luciferase Reporter (NL/1/00/A1 strain)

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the full length fusion (F) protein of Human metapneumovirus (HMPV) isolate NL/1/00/A1 (294E). HMPV is a respiratory virus with fusion (F) protein as the sole target of neutralizing antibodies. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices.

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pGEM®-T Easy Vector Systems, Promega®

pGEM®-T Easy Vector Systems, Promega®

Supplier: Promega Corporation

The pGEM-T Easy Vector Systems are convenient systems for cloning PCR products. They offer all of the advantages of the pGEM-T Vector Systems with EcoRI and NotI sites flanking the insertion site.

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pGL4.34[luc2P/SRF-RE/Hygro] Vector, 20 µg, Promega

pGL4.34[luc2P/SRF-RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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pGL4.51[luc2/CMV/Neo] Vector, 20 µg, Promega

pGL4.51[luc2/CMV/Neo] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4 Luciferase Reporter Vectors are the next generation of luciferase reporter vectors optimized for expression in mammalian cells.

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CheckMate/Flexi Vector Mammalian Two-Hybrid System, 1 each, Promega

CheckMate/Flexi Vector Mammalian Two-Hybrid System, 1 each, Promega

Supplier: Promega Corporation

The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BQ.1.1 Strain Spike with Luciferase Reporter

VSV-Pseudovirus_SARS-CoV-2 Omicron BQ.1.1 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BQ.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_SARS-CoV-2 Omicron XBB.1.5 Strain Spike with Luciferase Reporter

VSV-Pseudovirus_SARS-CoV-2 Omicron XBB.1.5 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron XBB.1.5. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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pNLF1-C [CMV/Hygro] Vector, 20 µg, Promega

pNLF1-C [CMV/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The three pNLF1 Vectors create N- or C-terminal fusions of NanoLuc luciferase and your protein of interest using a multiple cloning site (MCS). Insert your protein on the N or C terminus of NanoLuc luciferase or the N terminus of secreted NanoLuc enzyme.

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pNLF1-N [CMV/Hygro] Vector, 20 µg, Promega

pNLF1-N [CMV/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The three pNLF1 Vectors create N- or C-terminal fusions of NanoLuc luciferase and your protein of interest using a multiple cloning site (MCS). Insert your protein on the N or C terminus of NanoLuc luciferase or the N terminus of secreted NanoLuc enzyme.

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NanoBRET PPI Control Pair (p53, MDM2), 2 × 20 µg, Promega

NanoBRET PPI Control Pair (p53, MDM2), 2 × 20 µg, Promega

Supplier: Promega Corporation

The NanoBRET PPI Control Pair (p53, MDM2) is an optimized pair of vectors designed to measure the interaction of protein partners p53 and MDM2 using the NanoBRET assay.

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pNLCoI1[luc2-P2A-NlucP/Hygro] Vector, 20 µg, Promega

pNLCoI1[luc2-P2A-NlucP/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.

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pFC32K Nluc CMV-neo Flexi Vector, 20 µg, Promega

pFC32K Nluc CMV-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.

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