You searched for: Enzymes

Supplier: MP Biomedicals

Papain is used in dissecting solutions

Supplier: Enzo Life Sciences

Isolated from human plasma as prothrombin, and activated with Factor Xa, Factor Va, phospholipids, and calcium to yield a-thrombin.

Supplier: Corning

0.25% Trypsin in HBSS (Hank’s Balanced Salt Solution) without calcium and magnesium.

Supplier: Rockland Immunochemical

Collagenase Type 4

Supplier: Rockland Immunochemical

GST is purified by chromatography from E.coli preparations. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-GST antibody. Size: 100ug.

Supplier: MP Biomedicals

Soluble in water; Insoluble in ethanol, acetone and isopropyl alcohol.

Supplier: MP Biomedicals

One unit catalyzes the liberation of 1 mmole reducing group (calculated as maltose per minute at pH 6.9 at 20 °C).

Supplier: MP Biomedicals

Dissolves readily at 5 mg/mL in 0.05m sodium/potassium phosphate pH 7.0 giving a clear green solution.

Supplier: MP Biomedicals

Soluble in water; practically insoluble in alcohol and glycerol.

Supplier: Cytiva

A high specific activity, heat-labile alkaline phosphatase. Completely and irreversibly inactivated in Tris buffers at pH 8,0 to 8,5 by heating for 15 minutes at 65 °C. No further treatment is necessary.

Supplier: MP Biomedicals

One unit will hydrolyze 1 µmole of N-benzoyl-L-tyrosine ethyl ester per minute at pH 7.8 and 25 °C. Produced from 3× crystallized chymotrypsinogen.

Supplier: Sino Biological

Recombinant full-length human WIP1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Supplier: Cytiva

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).

Supplier: AGILENT TECHNOLOGIES, INC (CSD)

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL (formerly ProZyme). A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications). Supplied at a concentration of ≥ 2.5 U/mL. For a more concentrated formulation of ≥ 10 U/mL, which may be useful for native digestions, see GKE-5010B or GKE-5020B (EDTA-free).

Supplier: Enzo Life Sciences

Enzyme regulation and kinetics, comparative studies of substrate or inhibitor specificities, cleavage of target proteins. Assay or digest conditions can vary widely, but concentrations for the MMP enzymes can range between 10 and 300nM, or higher. Reaction temperatures can be between 25 and 37°C, and reaction times can range from 10 min to overnight, again depending on application and substrate. A typical assay buffer is 50mM HEPES, pH 7.0, 10mM CaCl2, 0.05% Brij-35. For more information, contact Enzo Life Sciences Technical Support.

Supplier: MilliporeSigma

Biological Activity: Contains 775 units of TdT; one unit is the amount of enzyme required to transfer 1.0nmol of dAMP from dATP to the 3' -OH terminus of d(A)50 in 60 minutes at 37 °C.

Supplier: MP Biomedicals

Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin. In the latter case, although histones restrict susceptibility to nuclease action, over a period of time nearly all chromatin DNA is acted upon. According to Mirsky and Silverman, this could result from the looseness of histone attachment to DNA. They found that lysine-rich histones more effectively block DNase access to DNA than arginine-rich histones. Billing and Bonner suggest that DNase attacks the histone-free strand of chromatin DNA. Schmidt, et. al.indicate that hydrolysis of the histone-free region of DNA strands accounts for the initial rapid action of the enzyme on chromatin. Bollum reports degradation of synthetic homopolymer complexes by DNase I. The intracellular functions of the enzyme are probably controlled by a DNase inhibitor, which according to Lazarides and Lindberg is actin.

Supplier: Enzo Life Sciences

Supplier: Enzo Life Sciences

Produced in E. coli.

Supplier: MP Biomedicals

Bromelain is a glycoprotein which is a highly active thiol proteinase (non-specific cysteine protease), which splits glycine esters but not lysine esters; it is also a protein-digesting and milk-clotting enzyme. Substrates susceptible to bromelain include all of the common protein materials such as gelatin, casein, gluten, collagen, elastin, globulins and muscle fiber protein.

Supplier: TCI America

Product Categories: Bioscience, Hydrolases

Supplier: TCI America

Product Categories: Bioscience, Glycohydrolase, Hydrolases

Supplier: TCI America

Product Categories: Bioscience, Glycohydrolase, Hydrolases

Supplier: Abcam

RNase R

Supplier: Thermo Scientific Chemicals

Uses: diagnostics and cofactor regeneration of NAD(P)H in reductive processes.

Supplier: MP Biomedicals

Collagenase is a protease which cleaves the triple-helical protein called collagen

Supplier: Enzo Life Sciences

DPP4 inhibitor.

Supplier: Rockland Immunochemical

Collagenase Type 4

Supplier: Enzo Life Sciences

Produced in E. coli. Consists of 44kDa alpha catalytic and 26kDa ß regulatory subunits as an alpha2ß2 tetramer.

Supplier: Enzo Life Sciences

Produced in E. coli.