Tissue culture buffers provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure present. Used predominately in biomolecular research, the dry and liquid substances have a balanced inorganic salt composition. Lower concentration options permit equilibration with air in a closed system, while higher salt concentration options keep gaseous phases in equilibrium. Cell growth is optimal when plated in a nutritional medium that has been buffered.

1X Phosphate Buffered Saline without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells.

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Corning® Tris, 10X Buffered Saline, Liquid, Corning

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range

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Dulbecco's Phosphate Buffered Saline (DPBS), Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

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Corning® EDTA, 0.5M pH 8.0, Corning

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity. The use of EDTA therefore allows for greater control over many experiments.

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Corning® Phosphate-Buffered Saline (PBS), 10X Concentrate, pH 7,4, Molecular Biology Grade

Supplier: Corning

Phosphate Buffered Saline (PBS) is used to maintain a physiological pH and osmolarity, two key factors in tissue culture and molecular biology research

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PBS (Endotoxin-Free), Enzo Life Sciences

Supplier: Enzo Life Sciences

Sterile and endotoxin-free PBS for use with TLRgrade™ or endotoxin-free grade reagents.

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Corning® Sodium Bicarbonate, Corning

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7.2 - 7.4 in a culture environment

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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

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1X PBS (Phosphate Buffered Saline), pH 7.4

Supplier: GROWCELLS

Balanced salt solution used in many biomolecular applications

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PBS 1X-Dulbecco's Phosphate Buffered Saline Solution

Supplier: FUJIFILM IRVINE SCIENTIFIC INC.

PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.

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HEPES Buffer Solution 1 M

Supplier: FUJIFILM IRVINE SCIENTIFIC INC.

Buffer to maintain pH between 7.2 and 7.6. Suitable for use in cell cultures outside of a CO₂ incubator

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HHBS [Hanks' Buffer with 20 mM HEPES] (100 ml), AAT Bioquest

Supplier: AAT Bioquest

Hank's Balanced Salt Solution with 20 mM Hepes buffer (HHBS) is used for a variety of cell culture applications, such as washing cells, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

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PBS 10X-Dulbecco's Phosphate Buffered Saline Solution

Supplier: FUJIFILM IRVINE SCIENTIFIC INC.

PBS 10X Dulbecco's Phosphate buffered saline solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.