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458 results for Protein Purification

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Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

MabSelect™ VL Protein L Resin, Cytiva

MabSelect™ VL Protein L Resin, Cytiva

Supplier: Cytiva

MabSelect™ VL resin with a redesigned protein L ligand for high binding capacity and improved alkaline stability. For affinity capture of antibody Fabs, bispecific, and other variants containing a kappa light chain.

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Glutathione Sepharose®, Abcam

Supplier: Abcam

Glutathione Sepharose® (ab193267) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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SOURCE™ 30Q Ion Exchange Chromatography Media, Cytiva

SOURCE™ 30Q Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

SOURCE™ 30Q is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.

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Superose™ 6 Gel Filtration Media, Prep Grade

Superose™ 6 Gel Filtration Media, Prep Grade

Supplier: Cytiva

Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.

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Q Sepharose™ High Performance Ion Exchange Chromatography Media, Cytiva

Q Sepharose™ High Performance Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.

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EAH Sepharose™ 4B Affinity Coupling Media, Cytiva

EAH Sepharose™ 4B Affinity Coupling Media, Cytiva

Supplier: Cytiva

EAH Sepharose™ 4B allows for the stable carbodiimide-based coupling of carboxyl groups to Sepharose® 4B via an 11-atom hydrophilic spacer arm that permits extremely stable coupling.

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CM Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

CM Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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DEAE Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

DEAE Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

DEAE Sepharose Fast Flow is a weak anion exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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Rabbit IgG Affinity Resin Kit, ImmunoReagents

Supplier: Immunoreagents

For the removal of cross-reactivity to rabbit immunoglobulins.

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NHS-Activated Sepharose™ 4 Fast Flow Affinity Coupling Media, Cytiva

NHS-Activated Sepharose™ 4 Fast Flow Affinity Coupling Media, Cytiva

Supplier: Cytiva

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

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Pierce™ Immunoprecipitation, Protein A/G Coated Plate IP Kit, Thermo Scientific

Pierce™ Immunoprecipitation, Protein A/G Coated Plate IP Kit, Thermo Scientific

Supplier: Invitrogen

Pierce™ Protein A/G Coated Plate IP Kit uses coated Protein A/G microplates to perform immunoprecipitation assays in 96-well plates without resins, beads, centrifugation or magnets.

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BSA Removal Kit (Gold/Europium labelling), Abcam

Supplier: Abcam

Our BSA Removal Kit - Nanoparticles (ab204912) is a simple one-step, 10 minute method that effectively separates BSA from the antibody.

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Pierce™ Immunoprecipitation, Direct IP Kit, Thermo Scientific

Pierce™ Immunoprecipitation, Direct IP Kit, Thermo Scientific

Supplier: Invitrogen

The Pierce™ Direct IP Kit uses an activated resin to covalently immobilize IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.

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rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]

Supplier: TCI America

The rSRL is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. This lectin preferably binds to [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] glycan epitopes by its two distinct carbohydrate-binding sites.
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rSRL-LecBeads (1 ml gel) shows a binding capacity of about 7.5 mg or more agalacto human Transferrin [agalacto hTF] through its binding to a terminal [GlcNAcβ(1-2)Man] glycan epitope on agalacto hTF.

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Pierce™ c-Myc-Tag Magnetic IP/Co-IP Kit, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Magnetic c-Myc-Tag IP/Co-IP kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of c-Myc fusion proteins or co-IP experiments using c-Myc-tagged bait proteins.

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Protein G Sepharose®, Abcam

Supplier: Abcam

Protein G Sepharose® (ab193259) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Con A Sepharose™ 4B Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method.

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Pierce™ Co-Immunoprecipitation Kit, Thermo Scientific

Pierce™ Co-Immunoprecipitation Kit, Thermo Scientific

Supplier: Invitrogen

Pierce™ Co-Immunoprecipitation (Co-IP) Kit provides covalent antibody immobilization and all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.

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Sepharose™ CL-6B Gel Filtration Base Matrix

Sepharose™ CL-6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ CL-6B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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Sephadex™ G-15 Gel Filtration Media, Cytiva

Sephadex™ G-15 Gel Filtration Media, Cytiva

Supplier: Cytiva

Sephadex G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.

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Superose™ 12 Gel Filtration Media, Prep Grade

Superose™ 12 Gel Filtration Media, Prep Grade

Supplier: Cytiva

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability

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Sepharose™ 4B Gel Filtration Base Matrix

Sepharose™ 4B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ 4B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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Sephadex™ LH-20 Gel Filtration Media, Cytiva

Sephadex™ LH-20 Gel Filtration Media, Cytiva

Supplier: Cytiva

Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.

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2D Clean-Up Kit, Cytiva

2D Clean-Up Kit, Cytiva

Supplier: Cytiva

The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.

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Immunoprecipitation kit, Abcam

Supplier: Abcam

Abcam's Immunoprecipitation Kit (ab206996) can be used to perform immunoprecipitation (IP) and Co-IP for functional studies of immunoprecipitated proteins/complexes and SDS-PAGE or western blot analysis of immunoprecipitated proteins and complexes.

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rPSL1a-LecBeads [for Siaα(2-6)Gal]

Supplier: TCI America

The rPSL1a is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Polyporus squamosus. This lectin preferably binds to a [Siaα(2-6)Gal] glycan epitope (It very slightly interacts with lactose.).
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rPSL1a-LecBeads (1 ml gel) shows a binding capacity of about 10 mg or more human Transferrin [hTF] through its binding to a terminal [Siaα(2-6)Gal] glycan epitope on hTF.

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Pierce™ Fab Micro Preparation Kit, Thermo Scientific

Pierce™ Fab Micro Preparation Kit, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce Fab Micro Preparation Kit uses immobilized papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc fragments and subsequently to purify the Fab using Protein A agarose.

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Pierce™ Direct Magnetic IP/Co-IP Kit, Thermo Scientific

Pierce™ Direct Magnetic IP/Co-IP Kit, Thermo Scientific

Supplier: Thermo Scientific Chemicals

The direct magnetic IP/Co-IP kit uses NHS-chemistry to covalently immobilize IP antibodies onto magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

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Pierce™ Avidin Agarose, Thermo Scientific

Pierce™ Avidin Agarose, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce Avidin Agarose can be used in a variety of applications for the affinity purification of biotinylated macromolecules.

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Proteasome Purification Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences

High integrity kit for purification of proteasome complexes.

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