You searched for: Protein Purification
Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Antibody Concentration And Clean-Up Kit, Abcam
Supplier: Abcam
The Antibody Concentration And Clean-Up Kit method allows the user to : -easily and quickly remove excess buffer from the antibody for a more concentrated antibody solution. -perform a simple buffer exchange to transfer the antibody into a more favorable buffer for conjugation
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SOURCE™ 30Q Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
SOURCE™ 30Q is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.
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Sephadex™ G-100 Gel Filtration Media, Cytiva
Supplier: Cytiva
Sephadex G-100 is useful for desalting and buffer exchange of very large biomolecules.
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Protein A Agarose (High Affinity), Abcam
Supplier: Abcam
Protein A Agarose (High Affinity) (ab193255) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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SOURCE™ 15Q Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
Mono-sized 15 um rigid beads gives low back pressure and high flow rates allowing for high productivity.
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CM Sephadex™ C-50 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
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Calmodulin Sepharose™ 4B Affinity Chromatography Media, Cytiva
Supplier: Cytiva
Calmodulin Sepharose™ 4B is calmodulin immobilised by the CNBr method to Sepharose™ 4B. Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells. This protein is involved in many cellular processes such as glycogen metabolism, cytoskeletal control, neurotransmission, phosphate activity and control of NAD⁺/NADP⁺.
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Superose™ 6 Gel Filtration Media, Prep Grade
Supplier: Cytiva
Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.
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Q Sepharose™ High Performance Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.
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Q Sepharose™ XL Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
Q Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose Fast Flow media.
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EAH Sepharose™ 4B Affinity Coupling Media, Cytiva
Supplier: Cytiva
EAH Sepharose™ 4B allows for the stable carbodiimide-based coupling of carboxyl groups to Sepharose® 4B via an 11-atom hydrophilic spacer arm that permits extremely stable coupling.
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SP Sepharose™ XL Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
SP Sepharose™ XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.
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Rabbit IgG Affinity Resin Kit, ImmunoReagents
Supplier: Immunoreagents
For the removal of cross-reactivity to rabbit immunoglobulins.
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CM Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
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DEAE Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
DEAE Sepharose Fast Flow is a weak anion exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
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CNBr-Activated Sepharose™ 4B Affinity Coupling Media, Cytiva
Supplier: Cytiva
CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.
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NHS-Activated Sepharose™ 4 Fast Flow Affinity Coupling Media, Cytiva
Supplier: Cytiva
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
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Sephadex™ G-10 Gel Filtration Media, Cytiva
Supplier: Cytiva
Sephadex G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.
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Pierce™ Immunoprecipitation, Protein A/G Coated Plate IP Kit, Thermo Scientific
Supplier: Invitrogen
Pierce™ Protein A/G Coated Plate IP Kit uses coated Protein A/G microplates to perform immunoprecipitation assays in 96-well plates without resins, beads, centrifugation or magnets.
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Antibody Concentration and Clean Up Kit for Latex & Europium, Abcam
Supplier: Abcam
The Antibody Concentration and Clean Up Kit for Latex & Europium kit (ab269965) removes small molecule buffer components from the antibody or protein prior to use in the conjugation reaction.
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Sample Diluent NBS
Supplier: Abcam
Sample Diluent NBS (ab221827) is part of the reagents, controls and accessories range. High-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates are available.
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Gel Filtration Calibration Kits, Cytiva
Supplier: Cytiva
Gel Filtration Calibration Kits are suitable for calibration of high-resolution gel filtration media and columns such as Superdex, Superose, and Sephacryl.
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BSA Removal Kit (Gold/Europium labelling), Abcam
Supplier: Abcam
Our BSA Removal Kit - Nanoparticles (ab204912) is a simple one-step, 10 minute method that effectively separates BSA from the antibody.
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Pierce™ Immunoprecipitation, Direct IP Kit, Thermo Scientific
Supplier: Invitrogen
The Pierce™ Direct IP Kit uses an activated resin to covalently immobilize IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.
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rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
Supplier: TCI America
The rSRL is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. This lectin preferably binds to [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] glycan epitopes by its two distinct carbohydrate-binding sites.
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rSRL-LecBeads (1 ml gel) shows a binding capacity of about 7.5 mg or more agalacto human Transferrin [agalacto hTF] through its binding to a terminal [GlcNAcβ(1-2)Man] glycan epitope on agalacto hTF.
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Pierce™ c-Myc-Tag Magnetic IP/Co-IP Kit, Thermo Scientific
Supplier: Invitrogen
Magnetic c-Myc-Tag IP/Co-IP kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of c-Myc fusion proteins or co-IP experiments using c-Myc-tagged bait proteins.
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Pierce™ F(ab')2 Micro Preparation Kit, Thermo Scientific
Supplier: Invitrogen
Pierce F(ab')2 Micro Preparation Kit uses immobilized pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab')2 fragments that retain antigen binding activity.
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Pierce™ Co-Immunoprecipitation Kit, Thermo Scientific
Supplier: Invitrogen
Pierce™ Co-Immunoprecipitation (Co-IP) Kit provides covalent antibody immobilization and all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.
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Protein G Sepharose®, Abcam
Supplier: Abcam
Protein G Sepharose® (ab193259) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Con A Sepharose™ 4B Affinity Chromatography Media, Cytiva
Supplier: Cytiva
Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method.